RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Species Reactivity
Rat
UniProt ID
Q62751
Protein Name
Iron-responsive element-binding protein 2
Tag
N-terminal His-tagged
Molecular Weight
41 kDa
Concentration
200 μg/mL (prior to lyoph)
Conjμgation
Unconjμgated
Purity
>95%
Source
Prokaryotic Expressed Recombinant
Usage
Applications
SDS-PAGE, WB, ELISA, IP
Additional Information
Endotoxin Level: < 1.0 EU per 1 μg (determined by the LAL method).
Reconstitute in ddH2O to the original concentration prior to lyophilization. If additional dilution is required, dilute further in the original base lyophilization buffer excluding stabilizers such as Sarkosyl, Proclin, Trehalose or Sucrose. Do not vortex. Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months. Avoid repeated freeze/thaw cycles.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.