ALAS2 Protein, Human (His) catalyzes PLP-dependent condensation of succinyl-CoA and glycine to produce aminolevulinic acid (ALA), CoA, and CO$_2$. This recombinant human ALAS2, expressed in E. coli with an N-6*His tag, significantly contributes to heme formation in erythropoiesis, with catalytic activity reaching 75-85% of isoform 1.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 63.5 kDa |
| Appearance | Lyophilized powder |
| Formulation | Lyophilized a 0.22 μm filtered solution of Tris-based buffer, 50% glycerol. |
| Species | Human |
| Source | E. coli |
| Accession | P22557 (Q50-A587) |
| Gene ID | 212 |
| Alternative Names | 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; ALASE; ASB |
| Tag | N-6*His |
| Shipping Conditions | Room Temperature |
| Storage Information | Stable at -20°C for 2 years. After reconstitution, store at 4°C for 1 week or -20°C (with carrier protein) for longer. Aliquot and freeze at -20°C/-80°C for extended storage. |
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