ATP5F1A Protein, Human (His), an important component of mitochondrial ATP synthase (Complex V), coordinates ATP production from ADP. This recombinant human ATP5F1A, expressed in E. coli with an N-6*His tag, forms the catalytic core of F-type ATPase to achieve ATP hydrolysis.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 56-59.2 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 0.1% SKL, pH 7.4. |
| Species | Human |
| Source | E. coli |
| Accession | P25705-1 (Q44-A553) |
| Gene ID | 498 |
| Alternative Names | ATP synthase alpha chain; ATP synthase alpha chain; mitochondrial; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit 1; cardiac muscle; ; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit; isoform 2; alpha subunit; ATP5A; Atp5a1; ATP5AL2; ATPA_HUMAN; ATPM; hATP1; HEL-S-123m; MC5DN4; mitochondrial; |
| Tag | N-6*His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Enzyme activity of this recombinant protein is currently under evaluation, so we cannot provide a guarantee. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet