BCAT2 Protein, Human (His, Strep) catalyzes the initial reactions in branched-chain amino acid catabolism (leucine, isoleucine, valine), essential for energy production and nitrogen balance. This recombinant human BCAT2, expressed in E. coli with N-Strep and N-6*His tags, is crucial for cellular processes.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 44.4 kDa |
| Appearance | Solution |
| Formulation | 50 mM HEPES (pH 7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol. |
| Species | Human |
| Source | E. coli |
| Accession | O15382 (A28-V392) |
| Gene ID | 587 |
| Alternative Names | BCAT2; Branched-chain-amino-acid aminotransferase; mitochondrial; BCAT(m); Placental protein 18; PP18 |
| Tag | N-StrepⅡ; N-6*His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Specific activity is 1920.85 pmol/min/μg, determined by its ability to convert 2 mM leucine and 0.5 mM alpha-ketoglutarate to alpha-ketoisocaproate and glutamate over 8 minutes at Room Temperature. |
| Shipping Conditions | Dry Ice |
| Storage Information | Store at -80°C for 1 year from the date of receipt. After opening, it's stable at -20°C for 3 months. For extended storage, freeze in aliquots at -80°C. Avoid repeated freeze-thaw cycles. |
Datasheet