CA5A Protein, Human (His), a mitochondrial carbonic anhydrase, catalyzes the reversible conversion of carbon dioxide to bicarbonate. This recombinant human CA5A, expressed in E. coli with N-Met and C-His tags, provides essential bicarbonate for mitochondrial enzymes, supporting critical metabolic reactions like the urea and Krebs cycles.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 33 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 50 mM NaCl, 0.05% Brij 35, pH 5.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
| Species | Human |
| Source | E. coli |
| Accession | P35218 (A40-S305) |
| Gene ID | 763 |
| Alternative Names | Carbonic anhydrase 5A, mitochondrial; Carbonic anhydrase VA; CA-VA; CA5 |
| Tag | C-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Specific activity is >500 pmol/min/μg, measured by its esterase activity. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet