Citrate Synthase/CS Protein, Human (sf9, His), a key enzyme in carbohydrate metabolism, catalyzes the conversion of oxaloacetate and acetyl-CoA into citric acid, initiating the TCA cycle. This recombinant human CS is expressed in Sf9 insect cells with a C-terminal His tag.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 46 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
| Species | Human |
| Source | Sf9 insect cells |
| Accession | O75390 (A28-G466) |
| Gene ID | 1431 |
| Alternative Names | Citrate synthase, mitochondrial; Citrate (Si)-synthase; CS |
| Tag | C-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Enzyme activity of this recombinant protein is currently under evaluation, so we cannot provide a guarantee. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet