PPIF Human Recombinant, a 21 kDa non-glycosylated polypeptide (198 aa, 30-207), was produced in E. coli with a 20-amino acid N-terminal His tag and purified via proprietary chromatography.
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
|---|---|
| Molecular Weight | 21 kDa |
| Appearance | Sterile Filtered Colorless Solution |
| Formulation | 20mM Tris-HCl pH-7.5, 10% glycerol and 1mM DTT. |
| Species | Human |
| Source | E. coli |
| Accession | P30405 |
| Gene ID | 10105 |
| Alternative Names | Oeptidylprolyl Isomerase F, PPIF, CYP-D, CYP3, SnuCyp-20, Peptidyl-prolyl cis-trans isomerase mitochondrial, Cyclophilin F, FLJ90798, MGC117207, peptidylprolyl isomerase F. |
| Biological Activity | Specific activity is >250 nmol/min/mg, defined as the amount of enzyme that cleaves 1 µmol of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 with chymotrypsin. |
| Shipping Conditions | Blue Ice |
| Storage Information | For use within 2-4 weeks, store the vial at 4°C. For longer periods, store frozen at -20°C. For extended long-term storage, we recommend adding a carrier protein (e.g., 0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
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