HTRA2/OMI Protein, Human (His), a serine protease, induces cell death by inhibiting BIRC proteins and activating caspases, or via a BIRC-independent, caspase-independent pathway. This recombinant human HTRA2/OMI, expressed in E. coli with a C-terminal His tag, also cleaves THAP5 during apoptosis; notably, isoform 2 lacks proteolytic activity.
| Purity | Greater than 87% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 36.5 kDa |
| Appearance | Solution |
| Formulation | 50 mM Tris, 0.3M NaCl, 1 mM DTT, 20% Glycerol, pH 7.8. |
| Species | Human |
| Source | E. coli |
| Accession | O43464 (A134-E458) |
| Gene ID | 27429 |
| Alternative Names | Serine protease HTRA2; Serine proteinase OMI; OMI; PRSS25 |
| Tag | C-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | HtrA2 demonstrates protease activity by cleaving bovine β-casein. Incubation of β-casein (0.2 mg/mL) with HTRA2/OMI Protein, Human (His) (0.02 mg/mL) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, resulted in >95% β-casein cleavage (SDS-PAGE). |
| Shipping Conditions | Dry Ice |
| Storage Information | Store at -80°C for 1 year from the date of receipt. After opening, it's stable at -20°C for 3 months. For extended storage, freeze in aliquots at -80°C. Avoid repeated freeze-thaw cycles. |
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