IVD Protein, Mouse (sf9, His), a vital component of the leucine catabolic pathway, facilitates the transformation of isovaleryl-CoA/3-methylbutanoyl-CoA into 3-methylbut-2-enoyl-CoA. This recombinant mouse IVD, expressed in Sf9 insect cells with an N-terminal His tag, also shows some capacity to oxidize saturated short-chain acyl-CoA thioesters.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 45.3 kDa |
| Appearance | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, pH 7.5, 150 mM NaCl, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
| Species | Mouse |
| Source | Sf9 insect cells |
| Accession | Q9JHI5 (H31-R424) |
| Gene ID | 56357 |
| Alternative Names | Isovaleryl-CoA dehydrogenase, mitochondrial; IVD |
| Tag | N-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Shipping Conditions | Room Temperature |
| Storage Information | Stable at -20°C for 2 years. After reconstitution, store at 4°C for 1 week or -20°C (with carrier protein) for longer. Aliquot and freeze at -20°C/-80°C for extended storage. |
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