WST-1 is a water-soluble tetrazolium salt. It induces intracellular mitochondrial dehydrogenase to perform an NADH-dependent enzymatic reaction, releasing a water-soluble methylbenzene product. WST-1 can be used to assess cell proliferation and cytotoxicity by measuring light absorption at 450 nm.
| Assay Platform | Microplate reader |
|---|---|
| Purity | 0.98 |
| CAS Number | 150849-52-8 |
| Formula | C19H11IN5NaO8S2 |
| Molecular Weight | 651.34 |
| SMILES | O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O |
| Appearance | Solid |
| Color | Light yellow to brown |
| Solubility | H2O: 87.5 mg/mL (134.34 mM, need ultrasonic). DMSO: 25 mg/mL (38.38 mM, ultrasonic and warming and heat to 80°C) |
| Shipping Conditions | Room Temperature |
| Storage Information | 4°C, sealed storage, away from moisture and light |
Datasheet