NAD-ME Protein, Human (N-His), a mitochondrial malic enzyme, catalyzes the NAD-dependent oxidative decarboxylation of malate to pyruvate. This recombinant human NAD-ME, expressed in E. coli with a C-6*His tag, is essential for cellular energy metabolism and the TCA cycle, linking metabolic pathways and balancing redox reactions.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 65kD |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4. |
| Species | Human |
| Source | E. coli |
| Accession | P23368-1 (L19-E584) |
| Gene ID | 4200 |
| Alternative Names | Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD+; dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD+; dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase |
| Tag | C-6*His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Enzyme activity is 5.54×10^3 U/mg protein, measured by its ability to catalyze the reduction of NAD+ to NADH, with the NADH increase rate monitored at 340 nm. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet