Neurolysin Protein, Human (His) is a multifunctional enzyme that hydrolyzes oligopeptides (neurotensin, bradykinin, dynorphin A). This recombinant human Neurolysin, expressed in E. coli with an N-terminal His tag, also modulates cannabinoid signaling by promoting vasopressin degradation.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 67 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
| Species | Human |
| Source | E. coli |
| Accession | Q9BYT8 (S38-P704) |
| Gene ID | 57486 |
| Alternative Names | Neurolysin, mitochondrial; Microsomal endopeptidase; MEP; NLN; AGTBP; KIAA1226 |
| Tag | N-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | Specific activity is >100 pmol/min/μg, determined by its ability to cleave the fluorogenic peptide substrate (7-methoxycoumarin-4-yl)acetyl Pro-Leu-Gly-Pro-D-Lys(2, 4-dinitrophenyl)-OH. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet