PDK4 Protein, Mouse (221a.a, His) is a key kinase that regulates glucose and fatty acid metabolism by phosphorylating PDHA1 and PDHA2. This recombinant mouse PDK4, expressed in E. coli with an N-6*His tag, inhibits pyruvate dehydrogenase activity, modulates metabolite flux, and inhibits aerobic respiration.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 30 kDa |
| Appearance | Lyophilized powder |
| Formulation | Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose. |
| Source | E. coli |
| Accession | O70571 (I138-S368) |
| Gene ID | 27273 |
| Alternative Names | Pdk4; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 4; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4 |
| Tag | N-6*His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Shipping Conditions | Room Temperature |
| Storage Information | Stable at -20°C for 2 years. After reconstitution, store at 4°C for 1 week or -20°C (with carrier protein) for longer. Aliquot and freeze at -20°C/-80°C for extended storage. |
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