PTRH2 Protein, Human (His), an enzyme with potential peptidyl-tRNA affinity, promotes caspase-independent apoptosis. This recombinant human PTRH2, expressed in E. coli with an N-terminal His tag, regulates transcriptional regulators AES and TLE1, contributing to apoptotic processes.
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 14 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol. |
| Species | Human |
| Source | E. coli |
| Accession | Q9Y3E5 (G63-Y179) |
| Gene ID | 51651 |
| Alternative Names | Peptidyl-tRNA hydrolase 2, mitochondrial; PTH 2; BIT1; CGI-147 |
| Tag | N-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | The ED50 is 0.1557 μg/mL for inhibiting A549 human non-small cell lung cancer cell chemoattraction, corresponding to a specific activity of 6.422×10^3 U/mg. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet