SMAC/Diablo Protein, Human (His) is a core apoptotic participant that activates caspases in the cytochrome c/Apaf-1/caspase-9 pathway. This recombinant human SMAC/Diablo, expressed in E. coli with a C-terminal His tag, antagonizes IAPs, inhibits BIRC6/bruce binding to caspases, and destabilizes XIAP/BIRC4.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 22 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4. |
| Species | Human |
| Source | E. coli |
| Accession | Q9NR28-1 (A56-D239) |
| Gene ID | 56616 |
| Alternative Names | SMAC; Diablo homolog, mitochondrial; DIABLO |
| Tag | C-His |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Biological Activity | In a functional ELISA, the ED50 is 0.328 μg/mL for Biotinylated XIAP binding to immobilized Human SMAC (10 μg/mL, 100 μL/well). |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet