TFAM Protein, Mouse (His, Myc) is critical for regulating mitochondrial transcription, binding to light chain promoters, and essential for basal mtDNA transcription. In the TFB2M/POLRMT initiation complex, this recombinant mouse TFAM, expressed in E. coli with N-10*His and C-Myc tags, recruits POLRMT and promotes promoter opening.
| Purity | Greater than 90 % as determined by reducing SDS-PAGE. |
|---|---|
| Molecular Weight | 34 kDa |
| Appearance | Lyophilized Powder |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. |
| Species | Mouse |
| Source | E. coli |
| Accession | P40630 (S43-H243) |
| Gene ID | 21780 |
| Alternative Names | Transcription factor A, mitochondrial; mtTFA; Testis-specific high mobility group protein (TS-HMG); Hmgts |
| Tag | N-10*His; C-Myc |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Shipping Conditions | Room Temperature |
| Storage Information | Store at -20°C for up to 2 years from the date of receipt. After reconstitution, it's stable at 4°C for 1 week or -20°C for longer (with carrier protein). For extended storage, freeze in aliquots at -20°C or -80°C. |
Datasheet