Product Name
Ferroportin/SLC40A1 HEK293T Cell Transient Overexpression Lysate
Alternate Names
Ferroportin, Ferroportin-1, FPN1, FPN1IREG1ferroportin 1, HFE4, HFE4ferroportin-1, IREG1, iron regulated gene 1, Iron-regulated transporter 1, member 3, MST079, MSTP079, MTP1, putative ferroportin 1 variant IIIB, SLC11A3, SLC11A3iron regulated gene 1, SLC40A1, solute carrier family 11 (proton-coupled divalent metal ion transporters), solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3, solute carrier family 40 (iron-regulated transporter), member 1, solute carrier family 40 member 1
Expression Host
HEK293T
Species
Human
Type
293T Cell Transient Overexpression
Gene
SLC40A1
Plasmid
pCMV-SLC40A1 full-length
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Background
Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1.
Disclaimer Note
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.