In Vivo Model Development for Periodic Fever Syndrome

Protheragen offers comprehensive in vivo model development services tailored for Periodic Fever Syndrome (PFS) research. Our advanced animal modeling platform is designed to accelerate the discovery and preclinical evaluation of novel therapeutics for PFS, providing researchers and pharmaceutical partners with robust, translationally relevant tools to support their drug development pipelines.

Periodic Fever Syndrome comprises a group of autoinflammatory disorders characterized by recurrent episodes of fever and systemic inflammation. Accurate animal models are essential for elucidating disease mechanisms and evaluating therapeutic strategies. At Protheragen, we utilize well-characterized species, including mice (C57BL/6, BALB/c, and genetically engineered strains) and rats, to recapitulate key pathological and immunological features observed in human PFS. These models offer high translational value, enabling the assessment of disease progression, immune responses, and therapeutic efficacy in a controlled, reproducible setting.

Genetic Models

Genetic models involve the use of transgenic or knockout mice that harbor mutations in genes commonly associated with human Periodic Fever Syndromes, such as MEFV (Familial Mediterranean Fever), NLRP3 (Cryopyrin-Associated Periodic Syndromes), and TNFRSF1A (TNF Receptor-Associated Periodic Syndrome). These models are generated using CRISPR/Cas9 gene editing or conventional transgenic techniques. The primary advantage is their ability to closely mimic the genetic and pathophysiological basis of human PFS, making them invaluable for studying disease mechanisms, biomarker discovery, and testing targeted therapies.

Chemically-Induced Models

Chemically-induced models are established by administering agents such as lipopolysaccharide (LPS), poly(I:C), or other pro-inflammatory stimuli to induce fever and systemic inflammation. These models allow for rapid, reproducible induction of fever episodes and inflammatory cascades, facilitating the study of acute disease flares and the efficacy of anti-inflammatory interventions. Their key advantages include ease of induction, controllable disease onset, and suitability for high-throughput screening of candidate compounds.

Dietary/Environmental Models

Dietary and environmental models simulate PFS by exposing animals to specific dietary triggers (such as high-fat or allergen-enriched diets) or environmental stressors (like temperature shifts or pathogen exposure) that precipitate fever episodes. These models are particularly useful for investigating gene-environment interactions and the influence of external factors on PFS pathogenesis. Their main strengths are the capacity to model complex, multifactorial disease triggers and to evaluate interventions targeting lifestyle or environmental modifications.

Protheragen delivers an end-to-end solution for in vivo Periodic Fever Syndrome model development, from model selection and custom generation to comprehensive phenotyping and therapeutic evaluation. Our service includes detailed study design, animal husbandry, and execution of experimental protocols under strict ethical and quality standards. Key efficacy endpoints measured include body temperature monitoring, frequency and duration of fever episodes, cytokine profiling (e.g., IL-1β, IL-6, TNF-α), hematological and biochemical analyses, histopathological examination of affected tissues, and survival analysis. Our analytical capabilities feature advanced immunoassays, flow cytometry, molecular biology techniques, and imaging modalities. Rigorous quality control measures, including genotyping, health monitoring, and validation of disease phenotype, ensure data reliability and reproducibility throughout the study.

Partnering with Protheragen gives you access to scientifically validated animal models, expert project management, and a collaborative approach that prioritizes your research objectives. Our commitment to quality, innovation, and client-focused solutions ensures that your PFS therapeutic development is supported at every stage. Contact us today to discuss your project needs and discover how our in vivo model development services can accelerate your path to breakthrough therapies.