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piRNA Array Service

Employing sophisticated technologies and efficient work processes, Protheragen reveals insights into piRNA's functions in gene regulation, genomic stability, and disease mechanisms through high sensitivity and high-throughput piRNA array analysis services. Our workflows and services are tailored for multiple species, allowing cross-species comparisons and species-specific studies.

Overview of piRNA Array

The piRNA array enables high-throughput analysis of expression profiling of piRNAs, a particular class of small non-coding RNAs that play important roles in gene regulation, transposon silencing, and maintenance of genome integrity. It serves as a cost-effective, efficient, and high-throughput means of piRNA research and circumvents the challenges posed by traditional sequencing methods. piRNA arrays, unlike common methods, are specifically designed to meet the difficulties that come with piRNA research.

Key Features of piRNA Array

Key Features of piRNA Array

  • High-throughput detection
  • High sensitivity and specificity
  • Cost-effective solution
  • Customizable design
  • User-friendly workflow
  • Integration with bioinformatics tools

Application of piRNA Array

The construction of piRNA arrays facilitates the rapid detection and quantification of piRNAs, making it possible to study their expression and functions. With these tools, researchers can probe deeper into the complexity of piRNA controlled regulatory networks and their impacts on health and disease.

  • piRNA profiling
  • Identification of novel piRNAs
  • Differential expression analysis
  • Functional studies
  • Biomarker discovery

Our Services

Protheragen remains an international leader in piRNA research by providing first-class services to all kinds of genomic researchers across the globe. Our advanced technology platforms allow us to offer a groundbreaking piRNA array service which can screen tens of thousands of piRNAs with unparalleled sensitivity and specificity. This service is designed to facilitate the exploration of piRNA biology, molecular functions, biomarkers, and disease therapeutic research.

Service Workflow

RNA isolation (optional)

RNA quality
control

Target reparation by labeling with Cy3

Array hybridization, washing, and scanning

Data extraction, analysis and summarization

Array Database

Scientists at Protheragen have developed piRNA arrays for human, mouse, and rat species to assess the overall expression of piRNAs.

Microarray Human piRNA Array Mouse piRNA Array Rat piRNA Array
Species human mouse rat
Reference genome GRCh38 MM10 RN6
Number of piRNAs 23,685 43,529 39,731
Probe length 60 nt 60 nt 60 nt
Labeling method Attachment of Cy3 label to dephosphorylated RNA using T4 RNA ligase.
Array format 4 * 44K 4 * 44K 4 * 44K

Our Advantages

  • Thorough compilation and dependable curation of piRNA sequences.
  • A 60-mer oligonucleotide probe structured in a double strand was employed for enhanced specificity.
  • Repeated probes for the majority of piRNAs.
  • High standards of quality control ensure reliable and consistent results.

Protheragen has developed a deep understanding of RNA research over the years and aims to provide complete piRNA analysis services tailored for academic, biotechnology, and pharmaceutical ventures. We are proficient in measuring piRNA expression levels through the utilization of high-throughput sequencing and microarray technologies. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

  1. He, Ling, et al. "Seminal plasma piRNA array analysis and identification of possible biomarker piRNAs for the diagnosis of asthenozoospermia." Experimental and Therapeutic Medicine 23.5 (2022): 1-12.