piRNA High-Throughput Sequencing Service

Protheragen provides comprehensive piRNA biology analysis support for your research needs with our piRNA high-throughput sequencing services. Our advanced sequencing technologies, flexible analysis workflows, and professional assistance make us your partner of choice for piRNA research development.

Introduction to piRNA High-Throughput Sequencing

Piwi-interacting RNAs (piRNAs) have distinct sequence patterns and lower expression levels than other small non-coding RNAs, which makes them quite challenging to work with. The development of high-throughput sequencing technologies, or next-generation sequencing (NGS), has greatly improved the efficiency and lowered the costs of genomic and transcriptomic research through advanced and rapid sequencing. These advancements serve as game changers to obstacles faced in piRNA research.

Fig 1 Schematic diagram of piRNA sequence analysis. (Barreñada, Odei, et al., 2021)

Application of piRNA High-Throughput Sequencing

High-throughput sequencing of piRNA is a relatively new method that offers unprecedented opportunities in analysing piRNAs in-depth, ranging from fundamental research and clinical investigations to even drug development. With high-throughput sequencing technology, researchers can study new areas of piRNA biology and elucidate the aetiologies of various diseases while aiding in the development of targeted molecular medicine for complex health issues.

Our Services

Protheragen provides advanced piRNA high-throughput sequencing services that incorporate next generation sequencing (NGS) technology to unravel the mystery of piRNAs. The services aid researchers in studying piRNA isoform expression, tissue-specific expression, disease associations, and even uncharacterized piRNAs that provide novel context for gene regulation, diagnosis, and disease therapeutics.

Service Workflow

Experimental Design

Our process begins with an initial joint meeting. Here, we define the study aims with you, give guidance on sample sizes, and advise on sequencing strategies. Customized workflows alleviate problems with low-input samples or transposon-focused analyses, ensuring statistically robust experimental design.

Sample Preparation

RNA is extracted using small RNA-optimized protocols, followed by electrophoretic or kit-based enrichment of 15-40 nt RNAs. Rigorous QC ensures high-quality input material, even for challenging samples like FFPE or biofluids.

Library Preparation

Adapter ligation (T4 ligase) and low-cycle PCR generate piRNA-specific libraries, with SPRI bead size selection (140–160 bp). Libraries undergo QC via Bioanalyzer and qPCR to confirm integrity and quantification prior to pooling.

High-Throughput Sequencing

Libraries are sequenced on Illumina platforms (NovaSeq/NextSeq) with single-end 50-75 bp reads, achieving >20 million reads/sample. Multiplexing with unique dual indexes ensures efficiency, while spike-in controls validate run performance.

Bioinformatics & Advanced Data Analysis

Raw data is processed via custom pipelines: adapter trimming, genome alignment, and annotation against piRNA databases. Differential expression, transposon silencing, and pathway enrichment analyses deliver actionable insights, with optional integration of multi-omics data.

Quality Control & Reporting

Multi-tiered QC includes technical replicates (Pearson R >0.98), spike-in controls, and independent validation (qRT-PCR/Northern blot). Final deliverables include raw data, publication-ready visuals (heatmaps, genome tracks), and a detailed report with biological interpretations.

Service Application

• Mapping piRNA expression across the genome
• Comparative analysis of piRNA expression levels
• Transposon element prediction and annotation
• Identify known piRNA and predict new piRNA

• Grouping analysis
• Anticipating new piRNA biomarkers
• Forecasting piRNA targets
• Genome-wide ORF and CDS annotation

Service Advantages

• Comprehensive piRNA profiling
• High sensitivity and accuracy
• High-resolution data
• Scalability and flexibility

• Integration with multi-omics approaches
• Cost-effectiveness
• Customizable analysis pipelines
• Expert support and guidance

Protheragen's piRNA high-throughput sequencing service can facilitate research endeavors involving genomics, epigenetics, developmental biology, and disease mechanisms. They can also help researchers understand the complex roles piRNAs play in health and disease. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.