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piRNA Chip Development Service

At Protheragen, we are focused on pushing the boundaries in piRNA research. We are pleased to offer the latest addition to our wide range of genomic services - the piRNA chip development service. This service enables researchers to elucidate the complex biology of piRNAs, including their multifaceted functions in gene regulation, transposon silencing, and the molecular pathogenesis of various diseases.

Introduction to piRNA Chip

The piRNA chip, or piRNA microarray, is a solid surface (most often a glass slide) containing thousands of microscopic spots, each of which has a specific DNA or RNA probe. These probes are designed to hybridize with complementary piRNAs in the sample. By measuring the intensity of the hybridization signal, the abundance and expression levels of specific piRNAs can be gauged.

chematic representation of piRNA profiles in cardiospheres, cardiosphere-derived cells, and cardiac fibroblasts. Fig 1 piRNA profiles in cardiospheres, cardiosphere-derived cells, and cardiac fibroblasts. (Vella, Serena, et al., 2016)

Applications of piRNA Chip

The piRNA chip is a customized high-throughput platform designed for piRNA detection and quantification in biological samples. Using the piRNA chip, researchers can profile piRNA expression on a genome-wide scale, thus elucidating their roles in genomic regulatory processes, transposon silencing, and overall genome maintenance.

Transposon silencing research
Epigenetic regulation research
Cancer biology research
Reproductive development research
Neurobiology research

Comparative genomics research
Functional genomics analysis
Therapeutic target identification
Biomarker discovery
Drug development

Our Services

Research on piRNAs has proven to be difficult because of the sophisticated processes involved and insufficient specialized tools. To help close this gap, Protheragen is committed to creating custom piRNA chips in an effort to supply researchers with a reliable high-throughput platform for piRNA analysis. Our goal is to make piRNA research as easy and efficient as possible so that scientists can devote their time to exploring and creating new things.

piRNA Chip Development Process

Steps	Description
Probe Design and Selection	Comprehensive sequences of piRNA are gathered from several databases and literature sources. Probes are tailored to ensure maximum sensitivity and specificity by finely optimizing length, GC content, and melting temperature to avert any chance of cross hybridization with other RNAs.
Probe Synthesis and Labeling	Once the probes are created, they will be made through high-quality oligonucleotide synthesis technology. Probes require modifications to improve binding affinity and stability. After that, they are marked with fluorescent dyes for visual and measurement purposes during hybridization.
Array Fabrication	The synthesized probes are deposited onto a solid surface, usually slides made of glass, by means of robotic spotting or using in situ synthesis approaches. Quality controls are performed to verify the integrity and consistency of the manufactured array.
Chip Validation	Check the array against known piRNA samples to validate probe-target binding specificity and find detection limits and chip dynamic range. Examine chip features by comparative analysis of hybridization experiments to facilitate further chip optimization and improvement.
Iterative Improvement	From the results of the validation, further refinement of the probe sequences, hybridization conditions, or fabrication of the array is carried out to ensure enhanced performance. The development of flawless and efficient piRNA chips for different types of research applications requires constant improvement.

Our Advantages

Comprehensive Sequence Coverage
Enhanced Specificity and Sensitivity
Robust Detection and Reliable Results

Customized Solutions and Flexibility
Cost-effective Workflow
Expertise and Innovation

At Protheragen, we aim to help fulfill the unique requirements of your research by providing end-to-end support that covers the entire scope from chip design to interpretation of the data. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

Vella, Serena, et al. "PIWI-interacting RNA (piRNA) signatures in human cardiac progenitor cells." The international journal of biochemistry & cell biology 76 (2016): 1-11.