piRNA Target Identification Service

Because piRNAs have remarkable properties in regulating gene expression and maintaining genomic integrity, as well as their specificity and therapeutic possibilities, piRNAs make appealing candidates for novel therapeutic approaches for different diseases. Protheragen's service on piRNA target identification will enable scientists to investigate the therapeutic uses of piRNA, as well as assist in the development and application of related pharmaceutical products.

Overview of piRNA Targets

As a result of its regulatory function on gene expression and genome stability, piRNA has garnered significant interest as a potential target for disease therapeutics. piRNA can be regarded as a feasible target in disease therapy for the following reasons:

Disease-associated piRNA Targets

piRNAs have emerged as extraordinary therapeutic targets for many prominent diseases, such as cancer, neurological and reproductive disorders, viral infections, cardiovascular diseases, and even metabolic disorders. Researchers could target certain piRNAs to develop novel therapeutics that treat the underlying pathologies of these diseases.

Our Services

Protheragen uses modern technology to provide efficient piRNA target identification services that allow researchers to investigate the therapeutic possibilities and molecular mechanisms of piRNAs. Our services are customized to identify and validate piRNAs with high specificity which help understand the role of piRNAs in regulatory processes, genome integrity, and disease progression. Regardless of cancer, neurodegenerative disorders, or infectious disorders, our unique offerings enhance your research by providing credible and useful information.

Service Workflow

Consultation and Experimental Design

Work with our specialists to create a personal study designed for your specific research objectives.

Sample Preparation

Isolate RNA from your specimens, including but not limited to tissues, cells, and biofluids.

piRNA Profiling and Target Prediction

Profile piRNA expression and assess potential targets with high-throughput piRNA sequencing, followed by data mining to predict targets.

Target Validation

Through functional assays including, but not limited to, luciferase reporter assay, qPCR, Western blot, validate predicted targets.

Data Analysis and Reporting

Conduction of thorough data analysis that encompasses target-pathway mapping, functional annotation, and visualization. Provide a report that includes valuable information and actionable insights.

Key Technologies and Methods

• piRNA Profiling: High-throughput sequencing, microarray analysis, qubit fluorometry, spike-in controls, annotation, etc.
• Target Prediction: Computational prediction tools (e.g., miRanda and TargetScan), Ping-Pong cycle analysis, crosslinking data integration, machine learning, functional enrichment, etc.
• Target Validation: Luciferase reporter assays, RNA immunoprecipitation, gene editing knockout, qRT-PCR, Western blot, cell proliferation/apoptosis assays, etc.
• Data Analysis: Statistical analysis (e.g., DESeq2/edgeR and Cytoscape), gene set enrichment analysis, survival analysis, multi-omics integration, etc.

Multiple Disease Research

Our scientists can identify piRNA targets for the therapeutic research of various diseases, including but not limited to the following:

Protheragen's piRNA target identification services help you discover key piRNA targets for diseases, paving the way for innovative therapy development. Contact us today to discuss your project and learn how we can support your research goals.