Human Ferritin Heavy Chain (FTH1) Chemi-Luminescent ELISA Kit
Reactivity
Human
Sensitivity
17.7 pg/mL
Detection Range
13.7-10,000 pg/mL
Description
The microplate provided in this kit has been pre-coated with an antibody specific to ferritin (fe). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ferritin (fe).
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates and Other Biological Fluids.
Components
FTH1 Microplate 96 Well strips
FTH1 Lyophilized Standard 2
100X FTH1 HRP-Detector Anitbody 120 µL
100X Avidin-HRP Conjugate 120 µL
Standard Diluent 20 mL
Detector Antibody Diluent 12 mL
Conjugate Diluent 12 mL
30X Wash Buffer 20 mL
100X Luminol Substrate 2 mL
Substrate Diluent 20 mL
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196).
Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196).
Disease Area
Hemochromatosis 5 (HFE5).
Storage & Handling
Storage
Store at -20 °C. Avoid repeated freeze-thaw cycles.
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