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Organoid Models Development Service

The development of organoid models can provide important information for drug research and development, the establishment of disease models, precision medicine, and regenerative medicine at the organ level. Our company has long-term devoted to the development and application of organoid models. We are pleased to use our extensive experience and one-stop technology platforms to offer the best service to satisfy each demand of our customers in rare disease research.

Overview of Organoid Models

Organoids refer to the use of adult stem cells (ASC) or organs pluripotent stem cells (PSC) in vitro self-assemble into 3D organ-type structures, which are very similar to the histological characteristics of human organs and can reproduce the physiological function of organs in vitro. Organoids have the characteristics of cell proliferation, differentiation, self-renewal, self-assembly, and long-term culture, and are an important bridge between traditional 2D culture and in vivo mouse models. Compared with traditional 2D culture models, organoids have many advantages, such as being closer to physiological cell composition and behavior, more stable genome, and more suitable for biological transfection and high-throughput screening.

1-3-2 Organoid Models Development Service-1Fig.1 Different differentiation platforms and organoid applications. (Sahu, S., and Sharan, S. K., 2020)

Organoid Models for Rare Diseases

Rare diseases pose significant challenges in terms of understanding their underlying mechanisms and developing effective treatments. Traditional research approaches often rely on animal models or cell lines, but these may not fully capture the complexity and nuances of rare diseases in humans. However, recent advancements in the field of organoid models have provided promising avenues for studying and advancing our knowledge of rare diseases.

Table 1 Organoid models for rare diseases. (Heydari, Zahra, et al., 2021)

Tissue/organ Rare Disease Cell source Outcomes and achievements Matrix
Brain Parkinson's disease iPSC An organoid-based model is presented that recapitulates the pathological features of lrrk2 associated with sporadic PD, which contributes to the progression of therapeutic discovery. Matrigel embedding
Retina Late-onset retinitis pigmentosa - The first phenotypically consistent late-onset retinitis pigmentosa model was established. This provides insight into the PDE6B-related mechanisms of RP. Matrigel suspension
Skin Atopic eczema - This study elucidates the mechanism by which FLG haploinsufficiency leads to atopic skin inflammation, contributing to understanding filaggrin-deficient phenotypes and the underlying molecular mechanisms associated with atopic skin inflammation. -
Cardiac Barth syndrome iPSC Organoids replicate the pathophysiology of Barth syndrome cardiomyopathy. This model provides new ideas for studying the pathogenesis of Barth syndrome and opens up new avenues for the treatment of Barth syndrome. -
Dilated cardiomyopathy - Engineered models show sarcomere dysfunction and impaired responses to mechanical and beta-adrenergic stress. This suggests that titin mutations cause dilated cardiomyopathy by disrupting the link between myogenesis and adaptive remodeling. Collagen solution
Duchenne muscular dystrophy iPSC In Duchenne muscular dystrophy-engineered cardiac organoids, we observed restoration of proper contractile function. This model provides a powerful tool for eliminating genetic causes and correcting muscle abnormalities. Collagen solution
Kidney Polycystic kidney disease iPSC This model recapitulates glomerular disease associated with podocyte epithelial injury and is proven to be a replicable and versatile model in regenerative medicine. Matrigel sandwich
Autosomal recessive polycystic kidney disease iPSC Patient-derived hiPSC mutant organoids exhibit a cystic phenotype that can be effectively prevented through gene correction or drug therapy. The discovery provides new avenues for studying kidney development and drug discovery. -
Congenital nephrotic syndrome - The expression of PODOCIN and NEPHRIN is reduced in the glomerular organoid model, providing a feasible method for screening podocyte toxicity. -
Lung Idiopathic pulmonary fibrosis iPSC-MSC The model faithfully reproduced IPF for drug screening. Alginate beads
Liver Wolman disease Patient-derived iPSCs Treatment of FGF19 in the culture system resulted in improved survival rates and reductions in lipid accumulation, ROS production, and tissue stiffness. Matrigel embedding
Alagille syndrome - Structural abnormalities in the biliary tree were successfully replicated in the simulation. Matrigel embedding
Colon and intestine Hirschsprung disease PSCs The model investigated the cellular and molecular mechanisms underlying Hirschsprung disease caused by a mutation in the PHOX2B gene. Matrigel embedding

Our Services

With the world's leading 3D culture technology, our company has successfully cultured a variety of organoid types such as lungs, stomach, intestines, liver, kidneys, etc. Our technical team provides comprehensive and customized organoid model development services to simulate the occurrence, progression, and treatment response of rare diseases, thereby accelerating the research and development of your rare disease therapies.

1-3-2 Organoid Models Development Service-2Matrix embedding
1-3-2 Organoid Models Development Service-4Single organoid aggregation
1-3-2 Organoid Models Development Service-5Static suspension
1-3-2 Organoid Models Development Service-6Agitated suspension
1-3-2 Organoid Models Development Service-7Spinning bioreactor
1-3-2 Organoid Models Development Service-8Organoid research
  • Source of Organoids
  • Adult stem cell (ASC) source
  • Embryonic stem cell (ESC) source
  • Types of Organoids
  • Brain organoid
  • Inner ear organoid
  • Retinal organoid
  • Skin organoid
  • Cardiac organoid
  • Kidney organoid
  • Lung organoid
  • Liver organoid
  • Pancreatic organoid
  • Gastric organoid
  • Intestinal organoid
  • Etc.
  • Applications of Organoids
  • Drug screening by organoid models
  • Toxicity testing by organoid models
  • Efficacy evaluation by organoid models
  • Organ-on-a-Chip

Why Choose Us?

As an integrated CRO, our company has extensive expertise in organoid model development for rare disease research. Whatever your specific requirements, we will work closely with you to develop a customized solution. We can quickly respond to the changing needs of your rare disease research projects. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

References

  • Sahu, S., and Sharan, S. K. "Translating embryogenesis to generate organoids: Novel approaches to personalized medicine." Iscience 23.9 (2020).
  • Heydari, Zahra, et al. "Organoids: a novel modality in disease modeling." Bio-design and Manufacturing 4 (2021): 689-716.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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