The test principle applied in this kit is sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ferritin, Heavy polypeptide (fth).
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids.
Components
Anti-Fth1 Microplate 96 Well strips
Fth1 Lyophilized Standard 2×10 ng
100X Biotinylated Fth1 Detector Antibody 120 µL
Avidin/HRP Conjugate 120 µL
Standard Diluent 20 mL
Detector Antibody Diluent 12 mL
Conjugate Diluent 12 mL
30X Wash Buffer 20 mL
TMB Substrate 9 mL
Stop Solution 6 mL
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196).
Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196).
Disease Area
Hemochromatosis 5 (HFE5).
Storage & Handling
Storage
Store at -20 °C. Avoid repeated freeze-thaw cycles.
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