Product Name
NDUFS7 HEK293T Cell Overexpression Lysate
Alternate Names
CI-20, CI-20kD, complex I, mitochondrial respiratory chain, 20-KD subunit, Complex I-20kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, FLJ45860, FLJ46880, MGC120002, MY017, NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial, NADH:ubiquinone oxidoreductase PSST subunit, NADH-coenzyme Q reductase, NADH-ubiquinone oxidoreductase 20 kDa subunit, PSST subunit, PSSTcomplex I 20kDa subunit
Expression Host
HEK293T
Applications Note
This product is intended for use as a positive control in Western Blot.
Species
Human
Type
Overexpression
Gene
NDUFS7
Protein Tag
C-MYC/DDK
Plasmid
RC203279
Buffer
RIPA buffer
Background
This gene encodes a protein that is a subunit of one of the complexes that forms the mitochondrial respiratory chain. This protein is one of over 40 subunits found in complex I, the nicotinamide adenine dinucleotide (NADH): ubiquinone oxidoreductase. This complex functions in the transfer of electrons from NADH to the respiratory chain, and ubiquinone is believed to be the immediate electron acceptor for the enzyme. Mutations in this gene cause Leigh syndrome due to mitochondrial complex I deficiency, a severe neurological disorder that results in bilaterally symmetrical necrotic lesions in subcortical brain regions.
Disclaimer Note
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
WB
Applications Note
This product is intended for use as a positive control in Western Blot.
Contents
You will receive 1 vial of lysate (100 μg), 1 vial of empty vector negative control (100 μg), and 1 vial of 2xSDS sample buffer (250 μL).
Storage
Store at -80 °C. Avoid freeze-thaw cycles.
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.