This product is intended for use as a positive control in Western Blot.
Species
Human
Type
Overexpression
Gene
PAPL
Protein Tag
C-MYC/DDK
Plasmid
RC211792
Buffer
RIPA buffer
Disclaimer Note
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Usage
Applications
WB
Applications Note
This product is intended for use as a positive control in Western Blot.
Contents
You will receive 1 vial of lysate (100 μg), 1 vial of empty vector negative control (100 μg), and 1 vial of 2xSDS sample buffer (250 μL).
Storage
Store at -80 °C. Avoid freeze-thaw cycles.
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.