FTH1 ELISA kit (rat) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for fth1 has been pre-coated onto a 96-well plate (12 x 8 well strips) and blocked.
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids.
Components
Anti-Fth1 Microplate 96 Well strips
Fth1 Lyophilized Standard 2×2 ng
Sample Diluent 20 mL
Fth1 Biotinylated Detector Antibody 120 µL
Detector Antibody Diluent 12 mL
HRP Avidin Conjugate 120 µL
Conjugate Diluent 12 mL
25X Wash Buffer 30 mL
TMB Substrate 10 mL
Stop Solution 10 mL
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196).
Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196).
Disease Area
Hemochromatosis 5 (HFE5).
Storage & Handling
Storage
Store at -20 °C. Avoid repeated freeze-thaw cycles.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.