The test principle applied in this kit is sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to transferrin (tf).
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids.
Components
Anti-Serotransferrin Microplate 96 Well strips
Serotransferrin Lyophilized Standard 2×20 ng
100X Biotinylated Serotransferrin Detector Antibody 120 µL
Avidin/HRP Conjugate120 µL
Standard Diluent 20 mL
Detector Antibody Diluent 12 mL
Conjugate Diluent 12 mL
30X Wash Buffer 20 mL
TMB Substrate 9 mL
Stop Solution 6 mL
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Storage & Handling
Storage
Store at -20 °C. Avoid repeated freeze-thaw cycles.
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