Product Name
PANK2 HEK293T Cell Overexpression Lysate
Alternate Names
C20orf48, EC 2.7.1.33, FLJ11729, FLJ17232, Hallervorden-Spatz syndrome, HARPFLJ11729, HPANK2, HSS, MGC15053, NBIA1, PANK2, pantothenate kinase 2, pantothenate kinase 2, mitochondrial, Pantothenic acid kinase 2, PKAN, PKANneurodegeneration with brain iron accumulation 1 (Hallervorden-Spatz syndrome)
Expression Host
HEK293T
Applications Note
This product is intended for use as a positive control in Western Blot.
Species
Human
Type
Overexpression
Gene
PANK2
Protein Tag
C-MYC/DDK
Plasmid
RC217228
Buffer
RIPA buffer
Background
PANK2 encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms.
Disclaimer Note
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications
WB
Applications Note
This product is intended for use as a positive control in Western Blot.
Contents
You will receive 1 vial of lysate (100 μg), 1 vial of empty vector negative control (100 μg), and 1 vial of 2xSDS sample buffer (250 μL).
Storage
Store at -80 °C. Avoid freeze-thaw cycles.
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.