Chronic Unpredictable Mild Stress Model
Stressful events not only cause depressive symptoms, but also greatly exacerbate existing depressive symptoms. The chronic unpredictable mild stress model is one of the widely used animal models for simulating stress in human life. The stress model is closer to the pathogenesis of human depression, which is durable and stable, and is an ideal and reliable animal model of depression.
Our company has extensive experience in stress modeling and behavioral assessment. We are committed to providing customers with high quality animal models that can stably simulate human stress, and provide reasonable model analysis indicators to provide customers with a complete set of solutions in the research of related diseases.
Introduction of The Chronic Unpredictable Mild Stress Test
The chronic unpredictable mild stress model was established by chronically administering experimental animals a variety of mild and unpredictable stress stimuli, resulting in a decrease in the animals' preference for sweetened water. The chronic unpredictable mild stress model uses the detection of pleasure deficits as the key to successful modeling and is a more realistic simulation of life, work stress, and other major depressive symptoms that humans encounter in their lives.
Experimental Flow
We take rats as an example and subject them to chronic, unpredictable mild stimulation for 20 days. The rats were treated with one stimulus each day from the daytime and nighttime, in a randomized order, without consecutive repetitions, so that the rats could not anticipate the appearance of the stimulus.
| Days | Daytime | Nighttime |
|---|---|---|
| 1 | Illumination | |
| 2 | Electric shock | Tilt cage |
| 3 | Shake the cage for 10 minutes | Wet wood chips |
| 4 | Bound for 2 hours | Flash |
| 5 | Odor 3 hours | Fasting |
| 6 | Electric shock | Overcrowding |
| 7 | Icy water swimming | Illumination |
| 8 | 3 hours of solitude | Tilt cage |
| 9 | Bound for 2 hours | Fasting |
| 10 | Odor 3 hours | Wet wood chips |
| 11 | Electric shock | 3 hours of solitude |
| 12 | Shake the cage for 10 minutes | Overcrowding |
| 13 | Bound for 2 hours | Flash |
| 14 | Odor 3 hours | Fasting |
| 15 | Icy water swimming | Tilt cage |
| 16 | Bound for 2 hours | Overcrowding |
| 17 | Shake the cage for 10 minutes | Wet wood chips |
| 18 | Odor 3 hours | Flash |
| 19 | Electric shock | 3 hours of solitude |
| 20 | 3 hours of solitude | Fasting |
Fig. 1 The experimental design and the chronic mild stress (CMS) procedures. (Czéh, 2018)
Model Analysis Indicators
We will select the appropriate behavioral test method for the customer to analyze the model according to the specific experimental requirements of our customer.
- Weight test.
- Sucrose Preference Test.
- Open field experiment.
Features of The Chronic Unpredictable Mild Stress Model
- Consistent with the characteristics of the predisposing factors of depression (long-term, chronic, low-intensity stressful stimulation).
- With good apparent validity, construct validity and predictive validity, it is the most recognized animal model of depression.
- Widely used to study the neurobiological mechanism of depression and is also the most commonly used animal model of depression in the development of antidepressants.
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Reference
- Czéh, B.; et al. Long-term stress disrupts the structural and functional integrity of GABAergic neuronal networks in the medial prefrontal cortex of rats. Frontiers in cellular neuroscience. 2018, 12: 148.