Von Frey Test
The Von Frey test is a widely used, standardized test for quantifying mechanical stinging in rodents. Our company is committed to providing customers with complete solutions for rodent pain measurement, including Von Frey test. We provide customized test solutions to facilitate your relevant researches according to your demands.
Introduction of The Von Frey Test
In the Von Frey test, experimental animals are placed individually in cages with mesh or other bottom-penetrating cages. The monofilament was stimulated vertically on the plantar surface of the hind paw of the experimental animal until it flexed for 2-5 seconds. If the rodent appears to retract its paws, lick or shake its paws, it is considered to have a positive response. Once these responses occur, the required pressure is expressed in g and the value is considered as a single threshold value.
Fig. 1 Schematic diagram of the Von Frey test.
Experimental Flow
- Habituation period
- The experimental animals were placed in a test chamber for one hour to acclimatize to the environment.
- 0.6 g von Frey filament was applied as the starting filament to stimulate the left hind paw of the mouse, using sufficient force until the filament bent and remained in contact for 1-2 seconds.
- The size of the filament for the next test is selected by the response of the mice to the previous filament. If no withdrawal response was observed then the next highest filament is tested. If there was a withdrawal response then the next lowest filament is tested.
- Continue testing with fine wire until each paw has been tested 5 times, at least 2 minutes apart each time.
- Continue testing with the right rear claw.
- Test period
- At the end of the habituation period, the mice were retested for pain at 24 h, 48 h, respectively, using the same method with von Frey filaments.
Applications
The following is an example of the development of mechanical allodynia induced by nerve injury.
Fig. 2 Development of mechanical allodynia induced by nerve injury. (Kim, 2019)
To confirm the development of mechanical abnormal pain, hind paw withdrawal thresholds in an animal model of neuropathic pain were examined using e-von Frey at 1, 4, 7 and 14 days after neuropathic pain surgery. Development of mechanical abnormal pain in nerve-injured (NP) and sham-injured (Sham) rats. After nerve injury, mechanical withdrawal thresholds were significantly lower in the NP group with POD 1, 4, 7 and 14 compared to the sham group.
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References
- Deuis, J. R.; et al. Methods used to evaluate pain behaviors in rodents. Frontiers in molecular neuroscience. 2017, 10: 284.
- Kim, K.; et al. Pain-relieving effect of mTOR inhibitors in the insular cortex of neuropathic rats. IBRO Reports. 2019, 6: S290.