Rearing Cylinder Test
The rearing cylinder test, also known as the limb asymmetric use experiment, focuses on the asymmetric use of limbs in rodents during the process of standing against the wall. The test has played an important role in the study of asymmetric central nervous system diseases.
Our company, a trusted provider of animal behavior tests, is committed to providing high-quality testing services and accurate data analysis services to help researchers save time and obtain scientifically reliable results in studies of movement disorders caused by neurological-related diseases.
Introduction of The Rearing Cylinder Test
In the rearing cylinder test, rodents are placed inside a clear glass cylinder and their locomotion is reflected by observing the preference of the animal to use its upper limbs in an upright position. Normally, rodents usually prefer to use both upper limbs to climb the cylinder wall by touching upward when performing exploratory behaviors. However, after limb injury, the frequency of using the injured side of the limb decreases significantly. Thus, the frequency and willingness to use the injured side of the limb can be assessed as a response to the rodent's recovery status.
Fig. 1 Rearing cylinder test.
Experimental Flow
- Three days prior to the start of the experiment, the animals are acclimated in a cylinder for 15-20 min and undergo baseline testing.
- During the formal experiment, the rodents were placed inside a transparent glass cylinder and the use of the limbs of the experimental animals during the exploration was recorded for 15 min.
- Each time the animal is upright, the upper limbs touch the cylindrical wall to complete (both upper limbs are marked with the ground) is recorded as a group. A total of the first 10 groups were analyzed, and the longest analysis time interval was 15min.
Our company is focused on providing high quality testing services to our customers. During the experiment, we will use the small animal behavior analysis system to video the whole exploration behavior of the experimental animals, and set up the glass behind the cylinder to obtain the situation when the animal's back to the camera.
Parameter Indexes
The analysis of the results of the rearing cylinder test was indicated by the first climb and sequential climbing. Therefore, there exist three different results for each climb using the left limb, the right limb or both limbs.
| Initial rear (The first use of upper limbs to climb the wall of a cylinder after the animal is upright.) | Left side (Climbing with left upper limb touch.) |
| Right side (Climbing with right upper limb touch.) | |
| Bilateral (Climbing with both upper limbs touching.) | |
| Subsequential rear (Exploratory climbing behavior that animals continue to make to the cylinder wall after the first climb.) | Left side (Behavior of animals continuing to climb the cylinder wall using the left upper limb after the first climb.) |
| Right side (Behavior of animals continuing to use the right upper limb to climb the wall of a cylinder after the first climb.) | |
| Bilateral (Continued climbing of the cylindrical wall by bilateral upper limbs in each sequence of consistent climbing). |
Applications
Rearing cylinder tests are important for the study of neurodegenerative diseases. We offer cylinder experiments to help our customers make progress in the study of the following related diseases.
- Alzheimer's disease.
- Parkinson's disease.
- Amyotrophic lateral sclerosis.
- Stroke.
Our company offers professional one-stop services from experimental design to results analysis to bring breakthroughs to your research. We guarantee on-time delivery of test results. If you need, you can contact us at any time.