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Tail Suspension Test

The tail suspension test is one of the classic experiments for antidepressant drug evaluation and is widely used in neuropharmacological studies.

Our company offers a variety of classical behavioral tests for the study of depression and the screening and evaluation of antidepressant drugs, including the tail suspension test. We focus on providing our clients with high quality testing solutions to support their researches in depression-related diseases and drugs.

Introduction of The Tail Suspension Test

The tail suspension test exploits the inability of experimental animals to overcome abnormal body positions and develop desperate behavior to assess the antidepressant effects of drugs. The device for the tail suspension test consists of a box with a hook at the top.

The test was performed by hanging the experimental animal upside down on the hook. Initially, the animal would struggle strongly to escape, but as it became clear that escape was not possible, the experimental animal would exhibit a depressive state of immobility. The depressive state was reflected by observing and recording a series of indicators during the experiment.

Tail suspension test in mice.Fig. 1 Tail suspension test in mice.

Experimental Flow

We provide an overview of the general process of the tail suspension test using mice as an example. Our experienced test team can provide customized test solutions to support your drug research according to your demands.

  • The mice were suspended upside down in the experimental box by taping to the tip third of their tails. The mouse's head was kept at a certain distance from the bottom of the hanging box.
  • The camera system was used to record the latency of the immobile state for 6 min and the duration of the immobile state for 4 min.

Parameter Indexes

During the experiment, we assessed depressive behavior by the duration of two types of behavior: immobility, and motor struggle. The longer the experimental animal remained immobility or struggled, the more severe the depression was.

Indexes Description
Immobility The animal stops struggling, the body stays upside down vertically and is stationary.
Struggle The animal has clearly visible struggling movements.

Our animal behavior analysis system videos the entire experiment and performs image tracking analysis to ensure scientific and reliable experimental results for our customers.


We provide our clients with high quality tail suspension test for depression-related disease and drug research applications, including but not limited to.

  • For rapid evaluation of the efficacy of antidepressants, stimulants and sedative drugs, such as tricyclics, selective serotonin reuptake inhibitors (SSRI), psychostimulants, tranquilizers, etc.
  • To assess the emotional behavior of animals such as anxiety, depression, etc.

Our company offers professional one-stop services from experimental design to results analysis to bring breakthroughs to your research. We guarantee on-time delivery of test results. If you need, you can contact us at any time.

For Research Use Only.