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Cold Hot Plate Test

The cold hot plate experiment is a pharmacological test to assess the effect of analgesics by observing the pain response of animals. Our company provides high quality hot and cold plate tests for our clients' pain mechanism researches and analgesic drug evaluations. With extensive testing experience and effective execution, we are committed to providing the most valuable testing solutions to complete your project.

Introduction of The Cold Hot Plate Test

When a person or animal is stimulated at a certain intensity, sensory signals are transmitted to the nerve center and then fed back to the motor center to cause a body response or protective reflex (limb retraction). In the cold hot plate test, the experimental animals are exposed to a cold or hot plate to make the animal feel acute painful stimulus. The animal's behavior in response to pain such as paw licking, jumping, etc. and the reaction time to pain can be used as reliable parameters to evaluate the analgesic effect of the compounds.

Hot plate and cold plate test.Fig. 1 Hot plate and cold plate test. (Deuis, 2017)

Many clinically approved drugs have been shown to delay the reaction time of rodent paws when exposed to thermal stimuli, including opioids and GABA enhancers. For example, when testing the efficacy of a certain analgesic drug, it is often compared between the control group and the administered group, the response time of the animals to heat-induced painful stimuli, if the animals in the administered group also have a significantly longer response time to the stimulus, indicating that the drug has an anti-injury perception effect or analgesic response.

We provide a brief description of the process of evaluating analgesic drugs using the hot plate experiment in mice as an example.

Analgesic activity research of drugs by hot plate test.Fig. 2 Analgesic activity research of drugs by hot plate test.

Parameter Indexes

We record a series of metrics during cold hot plate test and perform data analysis to assess the test potential of the compounds under test.

  • Forepaw retreating or licking.
  • Hind paws retreating or licking.
  • Stomp.
  • Tilt position.
  • Jumping.

Our company offers professional one-stop services from experimental design to results analysis to bring breakthroughs to your research. We guarantee on-time delivery of test results. If you need, you can contact us at any time.


  1. Deuis, J. R.; et al. Methods used to evaluate pain behaviors in rodents. Frontiers in molecular neuroscience. 2017, 10: 284.
For Research Use Only.