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Antigen Preparation Service

Obtaining high-quality antigens is one of the key factors for the success of antibody development. Our company can provide a variety of antigen preparation services, including peptide antigens, protein antigens, and DNA antigens, to help you make progress in the development of rare disease antibody therapies.

Two Characteristics of Antigens

The design and preparation of antigens are very important in the production process of antibodies. Antigens are the premise for the preparation of high-quality antibodies. To effectively produce vaccines and antibodies, antigens must have two characteristics:

  • Immunogenicity: antigens can induce immune responses in the immune system. Good immunogenicity can stimulate the body to produce humoral and/or cellular immune responses, thus producing antibodies.
  • Antigenicity: antigens can have specific reactions with the antibodies or effector cells they produce in vivo or in vitro.
2-2-4-1 Antigen Preparation Service-1Fig.1 Antigen-antibody preparation process (Tian, Wei, et al. 2018)

Protein antigens typically exhibit a more accurate conformation when compared to other forms such as peptides or DNA. Therefore, when a protein sample needs to maintain its natural conformation for testing purposes (e.g. development of neutralizing antibodies), protein antigen immunization is typically preferred. In cases where researchers encounter difficulties with full-length protein expression or require specific detection conditions, they can opt for alternative approaches such as using antigenic peptides or DNA.

Our Services

Antigens are key molecules that trigger immune responses, activate immune cells, and induce antibody production. Antigen preparation services are widely used in the diagnosis and treatment of rare diseases. Our company has a variety of antigen preparation platforms to meet your needs for different types of antigen preparation.

  • Peptide Synthesis

Synthetic antigenic peptides offer numerous advantages for polyclonal or monoclonal antibody generation and as components of peptide vaccines. These polypeptide antigens possess strong specificity, are easy to prepare, and can enhance antibody production more effectively.

Project Content
Peptide synthesis method
  • Solid-phase synthesis
  • Liquid-phase synthesis
  • Native chemical ligation
Sequence length
  • Up to 150 AA
Purity
  • Crude to ≥ 98%
  • TFA removal upon request
Additional analysis (upon request)
  • Solubility test
  • Water content analysis
  • Net peptide content analysis
  • Ion chromatography analysis (TFA, HAC)
  • Qualitative amino acid analysis
  • Solvent residue (DMF, ACN)
Modifications
  • Protein Expression

Protein antigens have a more complete conformation and are widely used in drug research and vaccine development. Our company offers a comprehensive range of solutions for recombinant protein expression. Our modalities for recombinant protein expression include, but are not limited to:

  • E. coli Expression System
  • Insect Expression System
  • Yeast Expression System
  • VLP-based Membrane Protein Expression
  • Mammalian Expression System
  • Inclusion Body Protein Renaturation

Please refer to the Recombinant Protein Development Platform page for more detailed information on our service process and cycles.

2-2-4-1 Antigen Preparation Service-2Gene synthesis
2-2-4-1 Antigen Preparation Service-3
2-2-4-1 Antigen Preparation Service-4Vector construction
2-2-4-1 Antigen Preparation Service-3
2-2-4-1 Antigen Preparation Service-5Protein expression
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2-2-4-1 Antigen Preparation Service-6Protein purification and QC analysis
  • Nucleic Acids as Antigen

DNA immune-technology and RNA immune-technology are reliable techniques for developing monoclonal antibodies targeting certain types of targets. They are outstanding tools for early research on DNA vaccines or RNA vaccines. Our immune technologies include, but are not limited to:

  • Hybridoma development via genetic immunization
  • Polyclonal antibody generation by genetic immunization
  • Immune antibody library via DNA immunization

Our company has successfully developed high-affinity antibodies through gene immunization services. The service process is as follows:

2-2-4-1 Antigen Preparation Service-7Codon optimization
2-2-4-1 Antigen Preparation Service-3
2-2-4-1 Antigen Preparation Service-2Plasmid preparation
2-2-4-1 Antigen Preparation Service-3
2-2-4-1 Antigen Preparation Service-8Animal injections
2-2-4-1 Antigen Preparation Service-3
2-2-4-1 Antigen Preparation Service-9Purification and sequencing

Our company has the capabilities and resources to provide professional communication and problem-solving support to ensure that we can quickly respond to the changing needs of your rare disease therapy research projects. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

  • Tian, Wei, et al. "Antibody production and application for immunoassay development of environmental hormones: A review." Chemical and biological technologies in agriculture 5.1 (2018): 1-12.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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