Gold Nanoparticle Development Service
Novel gene delivery systems based on gold nanoparticles (AuNP) are already being used as powerful tools for gene therapy to treat a wide range of diseases. Our company is committed to harnessing the unique properties of AuNPs to help our customers develop efficient and non-toxic non-viral gene delivery vectors. The advantages of these nanocarriers for gene editing and gene silencing applications are of great interest for the therapeutics of rare human genetic diseases.
Background
AuNPs offer unique optical properties, synthetic simplicity, and surface functionalization, and can be selectively co-modified with nucleic acid through covalent or non-covalent conjugation. Nucleic acid chains are covalently attached to the AuNP core via thiol groups. This strategy can be used for DNA and siRNA, which can be directly connected to gold cores or polymer-modified gold cores. The AuNP surface can be encapsulated by alternating anionic nucleic acid and polycationic layers (e.g. PEI or PAMAM) as well as by the addition of targeting ligands, so as to achieve specific interaction and binding of nanoparticles to cell surface receptors. However, the length, density, hydrophobicity, and affinity of ligands need to be considered and optimized to target nanoparticles effectively.
Fig. 1 Cas9/sgRNA delivery using AuNPs and gene editing. (Kanu G A, et al., 2022)
Our Services
Our researchers have established multiple protocols to help customers produce AuNPs with controlled size, shape, and surface properties. And we offer a variety of services for the development of AuNP constructs for gene delivery.
- Preparation of AuNPs
We help our customers prepare AuNPs through two methods: single-phase synthesis and two-phase synthesis. - Single-phase system by citrate reduction
- Two-phase system reduction followed by stabilization and functionalization by ligand exchange reaction, a method that allows for customization of multiple functionalities. We have made different modifications to this method to improve the application sensitivity and meet the various needs of customers by controlling the size, size distribution, shape, and functionalities.
- Functionalization of AuNPs for gene delivery
We help our customers to develop a wide range of AuNP constructs as non-viral vectors, which consist of monolayers of genetic material/or stabilizer molecules produced by covalent bonding or by electrostatic interactions. We synthesize different AuNPs constructs according to customers' needs, including nucleotide monolayer nanoparticles, cationic polymer monolayer nanoparticles, nucleotides and polymer-mixed monolayer nanoparticles, cationic lipid assembled nanoparticles, layer-by-layer assembled nanoparticles, and hydrophilic capped nanoparticles.
Our Advantages
- Unique expertise in bioconjugation chemistries
- Preparation of homogeneous AuNPs with well-regulated size and shape
- Optimized to meet your sensitivity specifications
- Small-scale to bulk manufacturing
Over the past decade, with advances in synthetic chemistry, bioconjugation chemistry, and molecular biology, our company has made considerable achievements in the design and development of AuNPs for gene delivery. We have established a variety of protocols to help our customers prepare a wide range of multifunctional AuNPs that promise to provide new avenues for gene therapy of rare diseases. If you require services for the development of AuNP-based gene delivery vectors, please contact us for more details.
Reference
- Kanu, G. A.; et al. Gold nanoparticle-mediated gene therapy. Cancers, 2022, 14(21): 5366.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
