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Single-stranded Circular DNA Therapy Development

Single-stranded circular DNA (circDNA) is a type of single-stranded DNA (ssDNA) with a covalently closed topology and is widely used in the development of DNA-based therapies. Our company offers comprehensive services and convenient solutions covering every stage of gene therapy development, designed to help our clients address possible barriers and challenges in the development of single-strand circular DNA therapy for rare diseases.

Overview of Single-stranded Circular DNA

Single-stranded circular DNA (circDNA), also called circular single-stranded DNA (cssDNA), is generally found in the mitochondria and chloroplasts of viruses, bacteria, and eukaryotes. Single-stranded circular DNA is generally more tightly bound and stable than linear DNA and can store more genetic information. Most DNA exists in a double helix structure, but it changes to a single-stranded state once it is treated with heat or alkali. Single-stranded DNA is different from double-stranded DNA in terms of molecular hydrodynamic properties, absorption spectra, and base reaction properties.

2-1-3-1-2 Single-stranded Circular DNA Therapy Development-1Fig.1 Schematic depiction of single-stranded circDNA therapeutics and diagnostics (theranostics). (Shen, T., et al., 2022)

Materials for DNA-based therapies have the advantages of low cost, biocompatibility, and simple chemical modification. In addition, the lack of terminals in circDNA prevents exonuclease degradation and enhances biological stability. Therefore, circDNA has been explored for application in various DNA-based therapies.

Applications of circDNA in DNA-based Therapy

circDNA Aptamers for Targeted Drug Delivery

Compared with monomeric aptamers, circDNA aptamers have higher serum stability and higher intracellular delivery efficiency. circDNA aptamers can be used to recognize homologous molecules and for binding to cellular targets.

circDNA for Sensitive Bioanalysis

Rolling circle amplification (RCA) is an isothermal nucleic acid amplification technique that enables the synthesis of long single-stranded nucleic acids through continuous and unidirectional replication of circular DNA templates. This process enables highly sensitive bioanalysis.

circDNA as Donors for CRISPR-Cas system

circDNA has the advantages of low off-target integration rate and high editing efficiency. It is considered the best donor in CRISPR-Cas system and can improve the editing efficiency of CRISPR-mediated gene knock-in, in vitro transcription and more experiments.

circDNA as Templates for Biomaterials Synthesis

Using circDNA as a template can synthesize DNA biomaterials, such as DNA nanoflowers, DNA origami, and DNA hydrogels, for a variety of biomedical applications.

Our Services

With years of rich experience in DNA-based therapy development and continuous improvement of technology platforms, our company provides the development of single-stranded circular DNA for targeted drug delivery, biomarker analysis, gene editing, and delivery material development. More importantly, our company provides a full range of one-stop single-stranded circular DNA therapy development services, enabling the research and development of rare disease therapies by DNA editing and repair related to rare diseases.

Single-stranded circular DNA Production

  • Scalable bacteriophage production of isogenic cssDNA
  • Shaker flask production of pure cssDNA
  • Batch fermenter production of pure cssDNA

Single-stranded circular DNA Testing

  • Agarose gel electrophoresis for purity testing
  • Sanger sequencing for equence accuracy
  • Spectrophotometer for optical density testing

Single-stranded circular DNA Delivery

  • Electroporation
  • Ultrasound
  • Gene gun

Why Choose Us?

With a research team with extensive expertise, our company is confident to provide clients with single-stranded circular DNA therapy development services for rare diseases. We have the capabilities and resources to provide professional communication and problem-solving support to ensure that we can quickly respond to the changing needs of your rare disease therapy research projects. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

  • Shen, T., et al. "Single-stranded circular DNA theranostics." Theranostics 12.1 (2022): 35.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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