Cell Viability Assay Service in Iron Metabolism Disorder
Iron is an essential micronutrient that sustains the life of living organisms. The process of iron metabolism has a well-controlled system in higher animals, including humans, to maintain a relatively balanced state of iron absorption and excretion. Protheragen offers a wide range of cell viability/activity assays to assist our customers in their iron metabolism related studies.
Cell Death Caused by Iron Metabolism Disorder
Iron is in a dynamic balance of constant absorption, utilisation, storage and circulation in the body, known as iron homeostasis. This homeostasis is regulated by multiple metabolic pathways as well as enzymes and organelles, and acute and chronic cellular stress resulting from abnormal metabolic and biochemical processes may trigger widespread non-apoptotic forms of cell death.
- Glutathione Depletion
Decreased glutathione peroxidase (GPX4) activity and reduced cellular antioxidant capacity lead to lipid peroxidation and metabolic dysfunction and increased lipid reactive oxygen species (ROS), which can trigger iron death.
Susceptibility to ferroptosis is closely linked to many biological processes, including amino acid, iron and polyunsaturated fatty acid metabolism, and the biosynthesis of glutathione, phospholipids, NADPH and coenzyme Q10, and is associated with pathological cell death associated with mammalian degenerative diseases, tumours, stroke, cerebral haemorrhage, traumatic brain injury, local ischaemia-reperfusion injury and renal failure.
Metabolic Processes Impinging on the Cell’s Susceptibility toward Ferroptosis [1].
Our Services
The viability of cells or cell populations is a reflection of overall cellular health, and Protheragen offers cell viability analysis services in the presence of disorders of patch metabolism to help our clients conduct in-depth studies of cell viability impairment due to disorders of iron metabolism.
Membrane Integrity Assay
We offer a variety of membrane integrity assays to help our customers perform in-depth analysis and studies of cellular activity in iron metabolism.
- Nucleic Acid Staining: Fluorescence is enhanced upon binding to exposed and double-stranded DNA (dsDNA) or RNA and is commonly used to detect dead cells.
- Membrane Integrity Reactive Dyes (Amino Dyes): Produce a very strong signal when the dye enters the cell through the damaged plasma membrane and reacts with amino proteins inside the cell.
Enzyme Activity Assay
We carry out studies of cell death due to abnormal iron metabolism by means of colourimetric methods and fluorometric methods used to quantify enzyme activity. Our customers can choose the appropriate experimental method for their research needs.
Mitochondrial Activity Assay
The homeostatic regulation of iron metabolism and iron-related apoptosis are very closely linked to mitochondria, and we offer a range of mitochondrial activity assays for monitoring changes in mitochondrial function by imaging and flow cytometry.
- Detection of Mitochondrial Superoxide Production
- Mitochondrial Calcium Ion Assay
- Mitochondrial Membrane Permeability Assay
- Detection of Dynamic Changes in Mitochondrial Membrane Potential
- Endpoint Detection of Mitochondrial Membrane Potential
Features
Advanced Technology
Experienced Team
High Quality and Efficiency
Best After-sales Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.
Reference
- Zheng J, et al. The metabolic underpinnings of ferroptosis[J]. Cell metabolism, 2020, 32(6): 920-937.