Cellular Iron Analysis Services
Iron is essential for many cellular processes, but cellular iron homeostasis must be maintained to ensure homeostasis of cellular signaling processes and to prevent disease. Protheragen provides assays related to intracellular iron metabolism to assist our clients with studies related to iron metabolism and iron homeostasis.
Cellular and Mitochondrial Iron Homeostasis
Cellular iron uptake is critical for meeting the cellular iron requirements of many metabolic processes, including electron transport, nucleotide synthesis, oxygen transfer, and redox reactions. The high redox potential of iron facilitates the reactions required for these processes, but it can also have pathological consequences, including increased mitochondrial reactive oxygen species (ROS) production and even cell death when iron homeostasis is disrupted. Therefore, precise cellular and mitochondrial iron uptake is essential for maintaining iron homeostasis and health.
Overview of cellular and mitochondrial iron homeostasis [1].
Cellular Regulation of Iron
Iron metabolism is regulated at both systemic and cellular levels. At the cellular level, regulation of the expression of proteins involved in iron metabolism and homeostasis in vivo, such as ferritin or transferrin receptors, is coordinated by the interaction of iron-sensing proteins, called iron regulatory proteins (IRP) or IRE-binding proteins. Thus, the IRP/IRE system is a critical component of the organism, enabling cells to rapidly accommodate cytoplasmic iron and promote the function of many iron-dependent cellular components at the post-transcriptional level.
Cellular iron regulation [2].
The intracellular iron pool is regulated by the binding of IRP1 and IRP2 to IRE. IRP1 and IRP2 are RNA binding proteins that interact with IRE to control the translation of proteins involved in iron metabolism. IRPs are either present at the 3′ UTR or the 5′ UTR of the target mRNA. When an IRP binds to a single IRE at the 5′ UTR, mRNA translation is repressed. On the other hand, the binding of IRP to IRE at the 3′ UTR stabilizes the transcript and leads to increased mRNA translation.
Our Services
Protheragen has been involved in research related to cellular iron metabolism for many years. With our complete assay platform and imaging equipment, we can provide a diverse range of cellular iron metabolism assays according to the different needs of our clients. We will tailor our technical solutions to meet the specific needs of our clients and solve all the problems identified in their cellular iron regulation studies.
Our Advantages
Advanced Technology
Experienced Team
High Quality and Efficiency
Best After-sales Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.
References
- Ali M Y, et al. Mitoferrin, Cellular and Mitochondrial Iron Homeostasis[J]. Cells, 2022, 11(21): 3464.
- Vogt A C S, et al. On iron metabolism and its regulation[J]. International journal of molecular sciences, 2021, 22(9): 4591.