ROS Detection Service

Reactive oxygen species (ROS-) induced lipid peroxidation plays a key role in ferroptosis, a basic and conserved mechanism based on excess ROS, which attack biological membranes, propagate lipid peroxidation chain reactions and subsequently induce different types of cell death. Protheragen offers assays related to reactive oxygen species in ferroptosis to help our customers conduct in-depth research on ferroptosis-related topics.

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ROS and Antioxidant System

Reactive oxygen species (ROS) are generated by normal physiological processes and play an important role in cell signaling and tissue homeostasis. However, excessive amounts of free radical substances can adversely modify cellular components and increase various pathogenic mechanisms such as lipid, protein and DNA damage.

• Generation of ROS
  ROS are partially reduced oxygen-containing molecules that are derivatives of free radicals and/or oxygen, including superoxide anions, hydrogen peroxide, hydroxyl radicals, lipid hydroperoxides, and peroxyl radicals. An imbalance in the rate of ROS production can lead to oxidative stress and consequent free radical production, which can damage DNA, proteins and lipids.
• Antioxidant System
  Antioxidants counteract free radicals and neutralize oxidants. The general endogenous antioxidant system consists of (1) enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), etc. and (2) non-enzymatic antioxidants, including vitamins or their analogues, etc.

Increase of ROS in Ferroptosis

Ferroptosis is a new form of programmed cell death characterized by an iron-dependent increase in ROS due to increased intracellular iron concentration and depletion of the antioxidant GSH leading to lipid peroxidation.

• GPX4 is a specific central regulator of Ferroptosis that protects cells and membranes from peroxidation by using glutathione as a cofactor for GPX4 to neutralize lipid peroxidation and protect membrane fluidity. Inhibition of GPX4 leads to increased ROS, whereas overexpression of GPX4 reduces ROS and subsequently prevents Ferroptosis.
• Iron levels in cells need to be strictly balanced, as excess iron can impair cellular function and eventually lead to cell death due to ROS production. increased ROS leads to lipid peroxidation and Ferroptosis, which can be inhibited by treatment with the iron chelator deferoxamine. In addition, higher levels of iron transport protein increase iron-mediated ROS, which subsequently leads to Ferroptosis. The production of ROS during iron metabolism mainly comes from the "Fenton reaction" and the Haber-Weiss reaction process.

Sources of ROS in ECs ^[1].

Our Services

ROS play an important role in cellular iron death, which leads to different cell fates. With our high-end cellular assay platform, we offer a wide range of ROS assays methods to help researchers in the study of lipid oxidation in ferroptosis.

ROS Detection Methods

Elevated ROS often accompanies iron death, a process that often leads to the development of multiple diseases. We provide assays for ROS levels during ferroptosis in animal or cellular models to assist our customers in disease research and drug development.

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Features

Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.