Iron Metabolism-related Protein Interaction Analysis

To maintain iron homeostasis at the systemic and cellular levels, vertebrates have developed a sophisticated mechanism to control iron uptake, storage, utilization and recycling. Protheragen offers a wide range of protein interaction assays to help our clients investigate new protein interaction mechanisms and provide new directions for studying the potential functions of proteins.

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Protein Interaction in Iron Metabolism

Iron is essential for the survival of almost all living organisms. However, inappropriately low or high levels of iron are harmful and can lead to a variety of diseases. Protein interactions form an important part of iron metabolism homeostasis, and in the last decade, a number of key mechanisms involved in iron regulation have been identified and a more complete picture of iron regulation is beginning to emerge.

• Iron Utilization, Recycling and Storage
  Erythroblasts acquire iron via a ubiquitous protein expressed on the cell surface, transferrin receptor 1, TfR1. Through receptor-mediated endocytosis, TfR1 transfers iron-loaded Tf (Holo-Tf) to acidified endosomes where iron is separated from transferrin with the help of six transmembrane prostate epithelial antigen (STEAP) proteins and leaves the endosome via DMT1. Transferrin and transferrin receptors are recycled back to the cell surface.

Hepcidin has a central role in maintenance of
iron homeostasis^[1].

• Whole-body Iron Homeostasis Regulation
  Intracellular and extracellular iron concentrations increase hepcidin transcription.
  The circulating peptide hormone hepcidin is a key molecule in the regulation of systemic iron homeostasis. hepcidin regulates iron efflux by binding to iron exporting iron transport proteins, triggering their internalization and degradation in the lysosome. this mechanism is facilitated by Janus kinase 2 (Jak2), which binds to the iron transport protein-ferroregulator complex, phosphorylates iron transport proteins, and  targets iron transport proteins for degradation.
• Intracellular Iron Homeostasis
  The iron regulatory proteins IRP1 (ACO1) and IRP2 (IREB2) regulate intracellular iron homeostasis post-transcriptionally in response to changes in iron levels. When intracellular iron levels are low, IRP binds to IRE with high affinity, and in iron-rich cells, the regulatory effect of IRP ceases.

Intracellular iron regulation^[2].

Our Services

Protheragen has been dedicated to protein-related research for many years and has a complete protein assay platform and equipment to provide diverse protein interaction assay solutions according to the different needs of our customers. We will customize our technical solutions to meet the specific needs of our customers to solve all their problems in iron metabolism-related protein interactions.

Pull-Down Protein Profile Identification

Through our mature pull-down detection process, we can complete protein interaction experiments quickly and accurately. Combined with our advanced UPLC-MS mass spectrometry platform, we have introduced a one-stop technical service for protein analysis based on pull down with high sensitivity and accurate resolution.

Protein Interaction Analysis

The regulation of iron metabolism is shared by multiple proteins, and we provide assays for multiple protein interactions to help our clients investigate the mechanisms of iron metabolism regulation.

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Features

Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related protein-based services. If you have any needs, please contact us.