Iron Metabolism-related Gene Analysis Services

Iron is essential for life, but it can also lead to cell death. As a result, cells have evolved a robust and tightly regulated set of genes to maintain iron homeostasis. Protheragen offers gene analysis services related to iron metabolism to help our clients conduct in-depth studies of gene function.

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Characteristics of Iron Metabolism-related Genes

Iron metabolism in cells consists mainly of uptake, utilization and efflux processes regulated by many genes and proteins. Genes related to iron metabolism consist mainly of iron uptake genes (TFRC and DMT1), utilization genes (FTH1, FTL, HIF1A, HMOX1, SLC25A37 and SLC25A38) and exporter genes (FLVCR1 and Fpn).

• TFRC
  TFRC is a direct downstream target of MYCN and mediates MYCN-induced GPX4-dependent iron death. Sp1 regulates TFRC transcription in cells by binding to the TFRC promoter region.

Potential binding sites for Sp1 within the TFRC promoter region ^[1].

Predicted iron-reactive elements
(IREs) in FTH1 ^[2].

• FTH1
  FTH1 is a major iron storage protein and plays a key role in important signalling pathways triggered by disruptions in iron and ROS levels. Also, downregulation of FTH1 is associated with tumourigenesis in breast and colorectal cancers, suggesting a tumour suppressive role for this key ferritin subunit that maintains iron homeostasis.
• Fpn
  Knockdown of Fpn accelerates erastin-induced iron death by increasing the accumulation of iron-dependent lipid ROS, which can be prevented by degradation of Fpn by Hepcidin.

Our Services

Protheragen have a complete gene analysis platform to provide diverse solutions for the study of genes related to iron metabolism, depending on the different needs of our clients. With our advanced DNA sequencing and RNA sequencing technologies, we help our clients better understand the differential expression of genes related to iron metabolism and meet the diverse needs of today's research laboratories.

Real-time Quantitative PCR/qRT-PCR Assay

We provide quantitative studies of mRNA transcript levels related to iron metabolism, determination of DNA copy number in different samples or genomes, validation of gene microarray results, and pharmacodynamic analysis.

mRNA-seq Analysis

We used mRNA-seq high-throughput sequencing technology to quantify the expression values of protein-coding mRNAs produced in a particular state of the test sample, thus providing a data base for comparison of samples in different states.

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Our Advantages

Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related gene assay services. If you have any needs, please contact us.