Iron Metabolism-related Gene Knock-out Service
Iron is essential for life, but it can also lead to cell death. As a result, cells have evolved a robust and tightly regulated set of genes to maintain iron homeostasis in animals. Protheragen offers gene knockout services related to iron metabolism to help our clients conduct in-depth studies of gene function.
Important Functions of Iron Metabolism Genes
Iron is an essential redox element that serves as a cofactor for many of the haemoglobin and iron-sulphur [Fe-S] proteins required to sustain essential life processes including DNA synthesis and repair, ATP production, cell cycle, oxygen transport and detoxification.
Developmental Effects of Iron Metabolism Gene Knock-out in Mice
The knockout of mouse iron metabolism genes that cause embryonic growth retardation or lead to embryonic death has proven to be a valuable tool in deciphering the prenatal iron regulation process and can help us understand the main features and mechanisms of iron metabolism in humans.
| Gene | Gene Product | Function | Knock-out Phenotype |
| TFR1 | Transferrin receptor 1 | Import of iron from transferrin into cells via endocytosis. | Increased lethality after knockout, affecting erythropoiesis and neurodevelopment. |
| slc11a2 | Divalent metal ion transporter protein 1 (DMT1) | Transports ferrous (Fe2+) and some divalent metal ions across the plasma membrane. | Transports ferrous (Fe2+) and some divalent metal ions across the plasma membrane. |
| slc40a1 | Iron transporter protein (Fpn) | Transports iron from the intracellular to the extracellular environment. | - |
| Hamp1 | Hepcidin | Central regulator of systemic iron homeostasis. Role in the regulation of the entry of iron into the circulation. | Reduction of hepatic iron content, decrease in hemoglobin concentration in liver-specific hepcidin KO embryos. |
| Hmox1 | Heme oxygenase 1 (HO1) | Role in in the enzymatic breakdown of heme molecules. | Abnormal placentation, inadequate remodeling of spiral arteries, intrauterine growth restriction, and eventually fetal lethality. |
Our Services
Gene knockouts are used in both classical genetics and modern techniques such as functional genomics. We offer Meganuclease, ZFNs, TALEN and CRISPR/Cas9 systems that can inhibit the function of a gene related to iron metabolism, depending on the customer's needs, and thus characterise the function of that gene.
Knock-out Cell Line Construction
We provide iron metabolism-related gene knock-out services for a variety of cell lines (A549, HCT116, HEK-293T, HeLa, etc.), and can accurately knock down specified genes. We help our clients to achieve efficient and flexible gene knock-out and to perform functional studies of iron metabolism-related genes.
Knock-out Animal Construction
Using custom iron metabolism knock-out animal models (mouse, zebrafish, rabbit, and rhesus monkey, etc.) we can help our clients discover gene function, explore cellular processes, and accelerate research on genetic mechanisms associated with iron metabolism.
Knock-out Plants Construction
We provide iron metabolism-related gene knock-out services in a variety of plants (Arabidopsis, cotton, maize, etc.), and can accurately knock out specified genes. We help our clients discover gene function, explore cellular processes, and accelerate research on genetic mechanisms associated with iron metabolism.
Workflow
Our Advantages
Advanced Technology
Experienced Team
High Quality and Efficiency
Best After-sales Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related gene assay services. If you have any needs, please contact us.