Iron Metabolism-related Gene Knock-in Service
Iron is essential for life, but it can also lead to cell death. As a result, cells have evolved a robust and tightly regulated set of genes to maintain iron homeostasis. Protheragen offers gene knock-in services related to iron metabolism in animals to help our clients conduct in-depth studies of gene function.
Iron Metabolism-related Genes
Iron is widely distributed in the body and clinical and experimental studies have shown that disturbances in iron metabolism in the central nervous system are associated with a number of neuropathies, such as Alzheimer's disease (AD) and Parkinson's disease (PD), but the exact mechanisms involved are not yet clear. Knock-in/knock-out of genes related to iron metabolism is often used to study the mechanisms involved in their association with disease.
- LRRK2
Missense mutations in the leucine-rich repeat kinase 2 (LRRK2) gene cause autosomal dominant Parkinson's disease (PD), and common genetic variants identified through genome-wide association studies are associated with idiopathic PD and inflammatory diseases. Research show that G2019S LRRK2 knock-in mice exhibit increased iron accumulation within microglia in the striatum in response to neuroinflammation compared to WT controls. These data support a role of LRRK2 in modulating normal iron uptake and storage in response to proinflammatory stimuli in microglia.
Schematic presentation of LRRK2 effects on ferroptosis and cellular metabolism [1].
- Rab8a
Rab8a has been shown to interact directly with the transferrin receptor (TfR) and regulate polarized TfR recycling to cell protrusions and transfer between cells through tunneling nanotubes. It was shown that mutant LRRK2 was able to isolate Rab8a, leading to transferrin mis-transport and intracellular iron accumulation.
Our Services
Protheragen provide customizable knock-in methods to integrate or replace exogenous functional genes into specific genomic loci according to customer needs and in obtaining Robinstably expressed cells or gene edited individuals. We help our clients with studies on gene function, disease models and drug screens related to iron metabolism.
Knock-in Cell Line Construction
We provide iron metabolism-related knock-in services for various cell lines (A549, HCT116, HEK-293T, HeLa, etc.) to help our clients achieve efficient and flexible knock-in services and conduct functional studies of iron metabolism-related genes.
Knock-in Animal Construction
Using custom iron metabolism knock-in animal models (mouse, zebrafish, rabbit, and rhesus monkey, etc.) we can help our clients discover gene function, explore cellular processes, and accelerate research on genetic mechanisms associated with iron metabolism.
Knock-in Plants Construction
We provide iron metabolism-related knock-in services in various plants (Arabidopsis, cotton, maize, etc.) to help our clients discover gene functions, explore cellular processes, and accelerate research on genetic mechanisms associated with iron metabolism.
Our Advantages
Advanced Technology
Experienced Team
High Quality and Efficiency
Best After-sales Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related gene assay services. If you have any needs, please contact us.
Reference
- Oun A, et al. LRRK2 protects immune cells against erastin-induced ferroptosis[J]. Neurobiology of Disease, 2022, 175: 105917.