Transferrin Assay Service
Transferrin is the major iron-containing protein in plasma, responsible for the transport of iron absorbed by the digestive tube and released by the degradation of red blood cells, and is an important indicator of the homeostasis of iron metabolism. Protheragen offers transferrin assay services to assist our clients with studies related to iron metabolism as well as iron homeostasis.
Transferrin
Transferrin (Tf) is an important β2 globulin that is an iron transporter in vertebrates. Different species of Tf have different physical, chemical and immunological properties, but all of them have two trivalent iron binding sites. In different studies, they are classified into common Tf or iron-saturated Tf, monoferric Tf and desferric Tf according to their iron content, common Tf and isomeric Tf according to their conformation.
Transferrin Classification[1].
Physiological Functions of Tf
Tf is an indispensable component of body fluids, not only involved in the transport and metabolism of iron, involved in the regulation of respiration, cell proliferation and immune system, but also can regulate iron ion balance and energy balance, moreover, it has the protective function of antibacterial and bactericidal, thus Tf has a more comprehensive protein physiological function. The main physiological function of Tf is to transport iron ions from the place of absorption and storage to the red blood cells for hemoglobin synthesis from absorption and storage to erythropoietic cells for hemoglobin synthesis or to other iron-requiring parts of the body.
Transferrin endocytosis and signaling pathways in protozoan parasites [2].
Tf Receptor (TR)
Many cells (placental tissue, reticulocytes, kidney tissue, tumor cells, etc.) contain TR on their surface. Iron forms Fe3+-Tf-CO32- complex with Tf through iron ion chelator, and then Fe3+-Tf-CO32- complex is adsorbed on the cell membrane by binding to TR on the surface of reticulocytes, which then enters the cell by endocytosis and releases iron, and then produces hemoglobin.
Our Services
Measurement of serum transferrin reflects the body's ability to transport iron as well as various diseases such as iron deficiency anemia. Protheragen measure transferrin by ELISA and RIA on serum samples provided by our customers. By analyzing the data, we help our clients to study iron transport-related mechanisms and diseases.
RIA Experiment Flow
Antigen-antibody Reaction
The antigen, labeled antigen and antiserum are reacted at a certain temperature for a certain time to bring the competitive inhibition reaction to equilibrium.
The Separation of B and F
The labeled antigen-antibody complex (B) formed in the RIA reaction does not precipitate on its own and requires a suitable precipitant to precipitate it completely.
Radioactive Intensity Assay
There are two types of measurement instruments, liquid scintillation counters (β-rays, such as 3H, 32P, 14C, etc.) and crystal scintillation counters.
Cellular transferrin levels can be used to diagnose a variety of diseases. We offer transferrin assays to assist our customers in disease research and drug development.
Our Advantages
Advanced Technology
Experienced Team
High Quality
Best Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.
References
- Parrow N L, et al. Lobe specificity of iron binding to transferrin modulates murine erythropoiesis and iron homeostasis[J]. Blood, The Journal of the American Society of Hematology, 2019, 134(17): 1373-1384.
- Reyes-López M, et al. Transferrin: Endocytosis and cell signaling in parasitic protozoa[J]. BioMed research international, 2015, 2015.