Lipid Peroxidation Assay Service
Ferroptosis is an iron-dependent, novel form of programmed cell death that is distinct from apoptosis, necroptosis, and autophagy, and is associated with the progression of many diseases. Protheragen offers assays for Lipid Peroxidation in ferroptosis to help our customers conduct in-depth research on ferroptosis related topics.
Lipid Peroxidation in Ferroptosis
Ferroptosis plays a crucial role in cell proliferation, senescence and differentiation. At the same time, iron levels in cells need to be strictly balanced, as excess iron can impair cell function and eventually lead to cell death due to ROS production. In addition, higher levels of iron transport proteins increase iron-mediated ROS, which subsequently leads to Ferroptosis. iron-mediated ROS is important for ferroptosis, and the production of ROS during iron metabolism mainly results from the "Fenton reaction" and Haber-Weiss reaction processes.
Lipid Peroxidation in Ferroptosis [1].
Ferroptosis Regulation
Ferroptosis can be triggered by structurally diverse small molecules or prevented by lipophilic antioxidants. Ferroptosis is caused by elevated levels of ROS due to elevated intracellular iron concentrations and depletion of the antioxidant GSH leading to lipid peroxidation, which leads to cell death.
- GPX4
GPX4 is an antioxidant enzyme that protects cells and membranes from peroxidation by using glutathione as a cofactor for GPX4 to neutralize lipid peroxidation and protect membrane fluidity. It is a specific and powerful central regulator of Ferroptosis when GPX4 is directly inhibited or indirectly inactivated by glutathione depletion. - GSH
GPX4 acts as an antioxidant by catalyzing its substrate GSH, therefore, GSH indirectly affects GPX4 activity. Meanwhile, Buthionine sulfoximine (BSO) induces ferroptosis by inhibiting glutathione levels. BSO is an inhibitor of glutamate-cysteine ligase, the rate-limiting enzyme of glutathione synthesis. - NRF2
NRF2 is a stress-inducible transcription factor whose protein levels are maintained at low levels by three different E3-ubiquitin ligase complexes. In disease settings with high NRF2 levels, such as cancer, tumor cells can use these protective detoxification systems to prevent lipid peroxide accumulation and lipid oxidation of target proteins to survive; whereas in diseases with low NRF2 levels, such as neurodegenerative diseases, increased lipid oxidation and inactivation of downstream NRF2 targets can significantly enhance overall protein lipid oxidation and Ferroptosis thereby promoting disease progression.
NRF2 target genes are involved in preventing lipid peroxidation and ferroptosis [1].
Our Services
Ferroptosis is characterized by excessive accumulation of lipid peroxides and reactive oxygen clusters. Protheragen provide fluorescence and colorimetric assays for the quantification of lipid peroxidation in cell and tissue culture supernatants and lysates, plasma, and other samples to help our customers conduct Ferroptosis-related studies.
Lipid Peroxidation Assay Methods
TBA
Malondialdehyde (MDA) is one of the lipid peroxidation products, which can interact with TBA (thiobarbituric acid) to produce a pink compound with a peak absorption at 532nm.
TBARS
TBARS can therefore be used as a better indicator of lipid peroxidation. The TBARS assay provides the ability to analyze levels of free radical activity in disease states and to detect the properties of many antioxidants.
Cellular Lipid Peroxidation Assay Indicators: Oxygen radicals, conjugated dienes, lipid peroxides, fluorescent products, aldehydes, hydroxylated gases.
One of the characteristics of ferroptosis is the massive accumulation of LPO, a physiological process that is closely linked to the course of many diseases. We offer assays for lipid peroxidation in a variety of ferroptosis-related disease models to assist our clients in pathology studies and drug development.
Features
Advanced Technology
Experienced Team
High Quality and Efficiency
Best After-sales Service
Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.
Reference
- Dodson M, et al. NRF2 plays a critical role in mitigating lipid peroxidation and ferroptosis[J]. Redox biology, 2019, 23: 101107.