Mitochondrial Membrane Potential Assay Service

Mitochondria play a critical and active role in cysteine deprivation-induced ferroptosis. Mechanistically, the mitochondrial TCA cycle and electron transport chain contribute to cysteine deprivation-induced ferroptosis by acting as a major source of cellular lipid peroxide production. Protheragen offers assays for mitochondria in ferroptosis methods to help our customers conduct in-depth research on ferroptosis-related topics.

---

The Pro-Ferroptosis Functions of Mitochondria

Mitochondria are double membrane-bound organelles that undergo oxidative phosphorylation and produce most of the energy in eukaryotic cells. The current study suggests that multiple metabolic activities in mitochondria have a more active role in the induction of Ferroptosis.

• Ferroptosis Caused by Mitochondrial TCA Cycle
  The mitochondrion houses the TCA cycle and various anaplerotic reactions that replenish the TCA cycle, such as glutaminolysis. Glutaminolysis inhibition or glutamine starvation was shown to significantly suppress ferroptosis induced by class 1 FINs. The potential mechanism by which the TCA cycle regulates ferroptosis may be related to its function in supporting electron transport and fatty acid biosynthesis.
• Functions of Mitochondrial Electron Transport Chain (ETC)
  Energy depletion activates the energy sensor AMP-activated protein kinase (AMPK), which promotes Ferroptosis by phosphorylating and inactivating acetyl coenzyme A carboxylase, while AMPK inactivation promotes Ferroptosis. Consistent with this, treatment with various ETC complex inhibitors or mitochondrial uncouplers significantly blocked class 1 FIN-induced Ferroptosis, suggesting that electron transport and proton pumping in mitochondria transport and proton pumps in mitochondria play an important role in the induction of Ferroptosis.

The role of mitochondria in promoting ferroptosis ^[1].

Mitochondrial Membrane Potential Abnormalities in Ferroptosis

During mitochondria-mediated apoptosis, a reduction in MMP is observed prior to final cell death. This reduction in MMP indicates mitochondrial outer membrane permeabilization (MOMP), an important feature of mitochondria-mediated apoptosis, and notably, multiple ferroptosis inducers can induce MMP hyperpolarization.

In summary, the ferroptosis-promoting effect of mitochondria may be attributed to ROS generated by mitochondrial respiration, which is dysregulated because of both the antioxidant system and the TCA cycle caused by glutamine catabolism.

Our Services

Mitochondrial membrane potential detection is an important method for detecting apoptosis, and Protheragen provides fluorescent probes that can rapidly and sensitively detect mitochondrial membrane potential changes in cells, tissues, or purified mitochondria for an in-depth study of the physiological process of ferroptosis.

• JC-l Mitochondrial Membrane Potential Assay
• TMRE Mitochondrial Membrane Potential Assay

Mitochondrial membrane potential is a functional mitochondrial parameter that reflects the level of cellular health and is associated with iron death as well as a wide range of diseases. We provide mitochondrial membrane potential assays for various cellular disease models according to the needs of our clients, helping researchers in pathology and drug development.

---

Features

Protheragen has a professional team and advanced equipment, and the whole process is operated by experienced technicians to provide our customers with iron metabolism related cellular assay services. If you have any needs, please contact us.