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Methotrexate (Abitrexate)

Product Name: Methotrexate (Abitrexate)
CAT#: DMME-2312-LGZ-2
Package: 100mg/200mg/500mg/10mM * 1mL in DMSO
CAS 59-05-2.png


Methotrexate is a cytotoxic agent that inhibits thymidylate synthetase and de novo purine synthesis.

Basic Information

Synonyms: MTX; Amethopterin; Abitrexate; Rheumatrex; Trexall
Application: DHFR Inhibitor
CAS Number: 59-05-2
Formula: C20H22N8O5
Molecular Weight: 454.44
SMILES: CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Solubility-In vitro: In vitro (25°C):
DMSO: Warmed: 89 mg/mL (195.84 mM)
Water: Insoluble
Ethanol: Insoluble
Solubility-In vivo: 2% DMSO+30% PEG 300+5% Tween 80+ddH2O: 4 mg/mL

Target Info

: Traditional Cytotoxic Agents


  • The lyophilized form should be stored at -20°C, in a desiccated environment.
  • In lyophilized form, the chemical remains stable for 36 months.
  • In solution, it should be stored at -20°C and used within 3 months to prevent loss of potency. Aliquoting is recommended to avoid multiple freeze/thaw cycles.

Preparing Stock Solutions

0.1 mM_1 mg: 22.01 mL 0.1 mM_5 mg: 110.03 mL 0.1 mM_10 mg: 220.05 mL 0.5 mM_1 mg: 4.4 mL 0.5 mM_5 mg: 22.01 mL 0.5 mM_10 mg: 44.01 mL 1 mM_1 mg: 2.2 mL 1 mM_5 mg: 11 mL 1 mM_10 mg: 22.01 mL 5 mM_1 mg: 0.44 mL 5 mM_5 mg: 2.2 mL 5 mM_10 mg: 4.4 mL

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