Advancements in biotechnology have been swift, with gene editing technology playing a pivotal role in the progression of the biological sciences in the 21st century. This progressive technology subtly remodels our grasp and implementation of life sciences.
Prominently, the CRISPR-Cas9 system has emerged as a popular tool in gene editing landscape. It facilitates precise DNA editing by employing guide RNA (sgRNA) to steer the Cas9 protein to clip designated DNA sequences. This CRISPR-Cas9 system possesses prospective benefits, including its elevated specificity, efficiency, and versatility. While other gene editing technologies such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and synthetic nucleases present unique features, they tend not to have as prevalent an application compared to the CRISPR-Cas9 system.
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