Gene Editing
- Knockout Kits (CRISPR)
- Transgene Knockin Kits
- CRISPRa Kits
- siRNA
Recently, biotechnology has made great strides, especially the rise of gene editing technology, which has become the key to progress in contemporary biosciences. Specifically, the CRISPR-Cas9 system is emerging as a transformative tool that is reshaping our understanding and utilization of the life sciences.
The system works by using guide RNA (sgRNA) to direct Cas9 proteins to precisely cleave specified DNA sequences. The key properties of the CRISPR-Cas9 system-high specificity, efficiency, and adaptability-suggest that it holds great promise for future applications. While other gene editing tools such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and synthetic nucleases offer unique advantages, they have not been as widely adopted as the CRISPR-Cas9 system in the current context.
Knockout Kits (CRISPR)
CRISPR-Cas9 is a naturally occurring defensive mechanism found in a wide range of bacteria. As a genetic engineering tool, CRISPR-Cas9 allows researchers to edit parts of the genome by adding, removing, or altering sections of the DNA sequence. A researcher can use this system to precisely target and cut any genomic locus, introduce a break in the double-stranded DNA.
Knockout kits are used in various sectors such as medical research, plant breeding, drug discovery and testing, as well as in studying the function of specific genes. They have revolutionized genetic engineering by making gene editing more accurate, faster, and more accessible.
Transgene Knockin Kits
The Transgenic Knockout Kit is a revolutionary step forward in our ability to detect and manipulate genetic information. This tool leverages genetic engineering techniques to specifically identify and remove targeted genes. It operates primarily through the processes of homologous recombination (HDR) and non-homologous end-joining (NHEJ) repair techniques; the introduction of the repair mechanism enables us to achieve precise gene knockout.
The prime function of these knockin kits is to provide researchers with a reliable means to explore gene function and regulation, develop models for disease, provide validation for therapeutic targets, or produce proteins that can be utilized for therapeutic purposes. Each kit generally includes essential components such as guide RNAs, transgenes, donor vectors, and other requisite materials for conducting a successful knockin experiment.
CRISPRa Kits
A CRISPRa (CRISPR activation) kit is a highly specialized tool used in the field of molecular biology that utilizes the sophisticated CRISPR technology to intentionally activate certain genes. This apparatus specifically zeroes in on distinct regions of the genome, triggering gene activation at these precise locations through the careful construction and arrangement of specific CRISPR-Cas complexes.
CRISPRa kits consist of all the necessary components needed to perform CRISPR-based gene activation experiments in cells. These kits can include deactivated Cas9 plasmid, guide RNA expressing plasmids, and other necessary elements for efficient CRISPRa.
siRNA
Small interfering RNA (siRNA) is a short-stranded RNA molecule, usually composed of 20 to 25 nucleotides. siRNA's main function is to regulate gene expression through the RNA interference (RNAi) mechanism.
When siRNA assembles with AGO proteins to form an RNA-induced silencing complex (RISC), one of the strands is degraded, while the other strand is complementarily paired with the mRNA of the target gene, resulting in the cleavage and degradation of the mRNA, thus achieving the purpose of inhibiting protein expression. This mechanism makes siRNA an effective tool for gene silencing, which can be used in the study of gene function, disease therapy and antiviral therapy.
